Start your experiments right - generate high-purity total RNA, mRNA, and miRNA using a wide array of Roche products for manual RNA preparation from a particular source or for certain applications, including formalin-fixed paraffin-embedded (FFPE) samples.
Precautions for handling RNA are critical for successful RNA analysis, such as gene expression analysis. The degradation of RNA by RNases must be avoided and if necessary, the RNA should be stabilized during storage and transport, or during the reverse transcription reaction.
Choosing the correct enzyme and reaction setup for reverse transcription and cDNA synthesis is critical for obtaining high yields of quality full-length cDNA with full gene representation. Roche offers a comprehensive selection of reverse transcription products:
Benefit from enzymes with consistent performance across a broad dynamic range.
Increase temperature to transcribe RNA with secondary structures.
Choose between well-known RTs (e.g., AMV, M-MuLV, the Transcriptor family, or RNase H- Expand Reverse Transcriptase), depending on your application.
Roche is committed to providing you with a multitude of high-performance choices in real-time PCR. LightCycler® Software and powerful Roche algorithms for qRT-PCR are available for capillaries, tubes, interchangeable 96- and 384-well plates, or the unique 1536-well format.
Roche enzymes and reagents provide accurate and efficient quantification of nucleic acids on a wide range of real-time PCR instruments. Obtain full-length cDNA transcripts, and achieve high reproducibility, even with difficult templates.
Use the GS FLX and GS Junior Systems to produce long sequencing reads, and follow up with the dedicated GS Assembler software for de novo assembly and comprehensive analysis of messenger RNAs (mRNAs) in the transcriptome of an organism.
Gene silencing by RNA interference starts with preparation of suitable siRNA or shRNA expression plasmids and transfection into cultured cells. Use Roche assays for subsequent RNAi effect analyses and quantification of mRNA and protein levels.
Find the most suitable enzymes and reagents to transcriptionally label your RNA probes using biotin, digoxigenin, alkaline phosphatase, and fluorescein. Use proven Roche kits for detecting DIG-labeled probes with alkaline phosphatase (AP), using colorimetric and chemilumiscent techniques, reagents for in situ analysis, and RNA molecular weight markers.
Roche provides high-quality enzymes and reagents for successful downstream analyses and characterization. Find enzymes to degrade cellular components, kits for nucleic acid isolation and purification, products for one- and two-step RT-PCR, proofreading enzymes for both cDNA and PCR, as well as polymerases for labeling nucleic acids. All kits and reagents are designed with minimal hands-on time: purification, cDNA synthesis, amplification, labeling, ligation, dephosphorylation, and transformation. Find everything you need for mapping and cloning of nucleic acids, including nucleases, markers agarose, vectors, and much more.
Catalog Products are: For life science research only. Not for use in diagnostic procedures unless otherwise indicated.
Custom Biotech Products are: For further processing only unless otherwise indicated.
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