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SP6/T7 Transcription Kit

For in vitro transcription of DNA.

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size List Price Quantity
1 kit for up to 2 x 20 transcription reactions $489.00
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Convenient kit for radioactive or nonradioactive labeling of RNA by the in vitro transcription with SP6 and T7 polymerase. The kit can also be used for "cold" transcription assays. By the in vitro transcription method single-stranded RNA probes of known length are produced, which can be used in a variety of hybridization techniques.


  1. pSPT18 DNA, 0.25 mg/ml
  2. pSPT19 DNA, 0.25 mg/ml
  3. Control DNA, (pSPT18- and pSPT19-neo-DNA, cleaved with Eco RI), 0.5 mg/ml
  4. ATP, in Tris buffer, 10 mM
  5. CTP, in Tris buffer, 10 mM
  6. GTP, in Tris buffer, 10 mM
  7. UTP, in Tris buffer, 10 mM
  8. Transcription Buffer 10x concentrated
  9. DNase I, RNase free, in buffer with 50% glycerol
  10. RNase Inhibitor, in buffer with 50% glycerol
  11. SP6 RNA Polymerase, in buffer with 50% glycerol
  12. T7 RNA Polymerase, in buffer with 50% glycerol 

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Roche Diagnostics Corporation
9115 Hague Road
P.O. Box 50414
Indianapolis, IN 46250-0414 USA

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