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Detection of High-Frequency and Novel DNMT3A Mutations in Acute Myeloid Leukemia by High-Resolution Melting Curve Analysis
The Journal of Molecular Diagnostics, 14, 4, Pages 336-345
A rapid high-throughput method for the detection and quantification of RNA editing based on high-resolution melting of amplicons
Nucleic Acids Res, 35, 17, e114 -
Transfering PCRs to HRM-assays on the LightCycler� 480 System- Examples for BRCA1
To evaluate how easy it is to design hrMCA assays using the LightCycler® 480 Instrument, we selected 3 different gene fragments, designed an MCA assay, and tested its sensitivity to detect known variants.
High Resolution Melting: Optimization Strategies
This technical note describes general steps of setting up HRM-based PCR assays, with a special focus on ways how to optimize procedures for gene scanning experiments
Rapid and Reliable Methylation Detection in Archival Tissue Samples using High-Resolution Melting Analysis
The aim of the present study was to establish and evaluate HRM assays for detection of promoter methylation on archival FFPE tissues from individuals with colorectal cancer.
Rapid High-Throughput Methylation Analysis Using the LightCycler� 480 System
We assessed the performance of the instrument combined with the LightCycler® 480 High Resolution Melting Master mix for DNA methylation analysis using the methylation-sensitive high resolution melting (MS-HRM) methodology.