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Total Search results for "reverse transcription" ()

To order these products, please contact your Roche order management team.

Products (15)

EvoScript Reverse Transcriptase

Easy-to-use reaction mix for reverse transcription

For general laboratory use.

EvoScript Universal cDNA Master

Easy-to-use reaction mix for cDNA synthesis for two-step RT-qPCR

For general laboratory use.

cDNA Synthesis System

Optimized one-tube procedure for the synthesis of double-stranded cDNA up to 3 kb from total RNA or mRNA.

For life science research only. Not for use in diagnostic procedures.

This link will direct you outside of Roche to our exclusive global distribution partners website.

Transcriptor High Fidelity cDNA Synthesis Kit

Transcriptor High Fidelity Reverse Transcriptase enzyme blend for high fidelity two-step RT-PCR of RNA up to 14 kb.

For life science research only. Not for use in diagnostic procedures.

This link will direct you outside of Roche to our exclusive global distribution partners website.

Transcriptor Reverse Transcriptase

A recombinant reverse transcriptase for the robust transcription of RNA fragments up to 14 kb used in two-step RT-PCR on thermal cyclers and real-time PCR instruments.

For life science research only. Not for use in diagnostic procedures.

This link will direct you outside of Roche to our exclusive global distribution partners website.

Publications (9)

Sensitive and Specific Quantitative Detection of Rotavirus A by One-Step Real-Time Reverse Transcription-PCR Assay without Antecedent Double-Stranded-RNA Denaturation 

Keywords: MagNA Pure 96 ,MagNA Pure Compact

S. Mijatovic-Rustempasic , K. I. Tam , T. K. Kerin , J. M. Lewis , R. Gautam , O. Quaye , J. R. Gentsch and M. D. Bowen

J Clin Microbiol, 2014, 52, 8

Use of the MagNA pure LC automated nucleic acid extraction system followed by real-time reverse transcription-PCR for ultrasensitive quantitation of hepatitis C virus RNA 

Keywords: MagNA Pure LC ,Probe

L. Cook , K. W. Ng , A. Bagabag , L. Corey and K. R. Jerome

J Clin Microbiol, 42, 9, 4130-6

Validation of Miniaturized One-Step Reverse Transcription qPCR Assays for High-Throughput Screening and Comparison to a Reporter Gene Methodology 

Keywords: Light Cycler® 480 ,Light Cycler® 1536 ,RealTime ready Cell Lysis kit ,RealTime ready RNA Virus master

C. Bardelle , L. McWilliams , S. Mounfield , M. Wigglesworth , K. Rich

94-101. doi:10.1089/adt.2014.630

Rapid quantitative detection of TE-AML1 fusion transcripts in pediatric acute lymphoblastic leukemia by real-time reverse transcription polymerase chain reaction using fluorescently labeled probes 

Keywords: LightCycler DNA Master Hybridization Probes ,LightCycler FastStart DNA Master Hybridization Probes

P. Bolufer , E. Barragan , A. Verdeguer , J. Cervera , J. M. Fernandez , I. Moreno , E. Lerma , C. Esquembre , M. Tasso , V. Fuster , M. Bermudez and M. Sanz

Haematologica, 87, 1, 23 - 32

High-Throughput RT-PCR for small-molecule screening assays 

Keywords: LightCycler 480 ,LightCycler 1536 ,RealTime ready cell lysis ,Transcriptor First Strand Synthesis Kit ,Roche SYBR Green Master ,Roche Probes Master ,Roche RNA Probes Master

J. A. Bittker

Curr Protoc Chem Biol 4, 1, 49-63

Documents (15)

Detection of Tumor-associated Antigen Gene Expression in Melanoma Tissues

A fast method to enrich rare mRNA templates for Reverse Transcription-PCR (RT-PCR)

(["Article"], PDF, 367 KB) Download

Transcriptor High Fidelity cDNA Synthesis Kit - Accuracy Meets RT-PCR

The kit not only reduces the amount of errors in RT-PCRs, it also allows the reverse transcription of full-length cDNAs with high yield.

(["Product Review"], PDF, 184 KB) Download

Biochemica 3.2005

We investigated the transcriptional organization of this cluster by RT-PCR analysis using the Transcriptor Reverse Transcriptase with primers that allowed us to detect co-transcription between genes.

(["Article"], PDF, 215 KB) Download

Transcriptor Universal cDNA Master

Reduce your reverse transcription time to 20 minutes by using the Transcriptor Universal cDNA Master - a convenient, ready-to-use master mix for fast cDNA synthesis in two-step real-time RT-PCR.

(["Article"], PDF, 2002 KB) Download

Improved Performance of the LightCycler® FastStart DNA MasterPLUS SYBR Green I Kit in Highly Sensitive Transcript Quantification

Real-time reverse transcription-PCR (RT-PCR) is considered the method of choice for any experiments requiring sensitive, specific, and reproducible quantification of mRNA molecules of interest. It is an essential tool for basic research, molecular medicine, and biotechnology.

(["Article"], PDF, 291 KB) Download
Product Information (2)

Roche Life Science | Products

Explore our complete portfolio of trusted products for working with DNA, RNA, cells and proteins.

Roche Life Science | Techniques And Applications

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