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Products (2)

RealTime ready Human Reference Gene Panel

Panel of qPCR assays for use on the LightCycler^® 480 Instrument to quantify expression of 19 human reference genes.

For life science research only. Not for use in diagnostic procedures.

RealTime ready Human Apoptosis Panel, 96

Panel of qPCR assays for use on the LightCycler^®480 Instrument to perform gene expression profiling of 84 human genes involved in apoptosis.

For life science research only. Not for use in diagnostic procedures.

Publications (1)

Resveratrol analogue 3,4,4?,5-tetramethoxystilbene inhibits growth, arrests cell cycle and induces apoptosis in ovarian SKOV?3 and A-2780 cancer cells

Keywords: Cell Death Detection ELISA PLUS kit ,RealTime ready Human Apoptosis Panel 96 ,LightCycler 480 Probes Master kit ,Universal Probe Library

H. Piotrowska , K. Myszkowski , A. Ziolkowska , K. Kulcenty , M. Wierzchowski , M. Kaczmarek , M. Murias , E. Kwiatkowska-Borowczyk , J. Jodynis-Liebert

Toxicol Appl Pharm, 263, 1, 53-60

In the screening studies, cytotoxicity of 12 methylated resveratrol analogues on 11 human cancer cell lines was examined. The most active compound 3,4,4′5- tetramethoxystilbene (DMU-212) and two ovarian cancer cell lines A-2780 (IC₅₀ = 0.71 μM) and SKOV-3 (IC₅₀ = 11.51 μM) were selected for further investigation. To determine the mechanism of DMU-212 cytotoxicity, its ability to induce apoptosis was examined. DMU-212 arrested cell cycle in the G2/M or G0/G1 phase which resulted in apoptosis of both cell lines. The expression level of 84 apoptosis-related genes was investigated. In SKOV-3 cells DMU-212 caused up-regulation of pro-apoptotic Bax, Apaf-1 and p53 genes, specific to intrinsic pathway of apoptosis, and a decrease in Bcl-2 and Bcl 2110 mRNA expressions. Conversely, in A-2780 cells an increased expression of pro-apoptotic genes Fas, FasL, TNF, TNFRSF10A, TNFRSF21, TNFRSF16 specific to extracellular mechanism of apoptosis was observed. There are no data published so far regarding the receptor mediated apoptosis induced by DMU-212. The activation of caspase-3/7 was correlated with decreased TRAF-1 and BIRC-2 expression level in A-2780 cells exposed to DMU-212. DMU-212 caused a decrease in CYP1A1 and CYP1B1 mRNA levels in A-2780 by 50% and 75%, and in SKOV-3 cells by 15% and 45%, respectively. The protein expression was also reduced in both cell lines. It is noteworthy that the expression of CYP1B1 protein was entirely inhibited in A-2780 cells treated with DMU-212. It can be suggested that different CYP1B1 expression patterns in either ovarian cell line may affect their sensitivity to cytotoxic activity of DMU-212.

Documents (8)

Monitoring Gene Expression Changes in Human Multipotent Mesenchymal Stromal Cells using RealTime ready Assays

Using the RealTime ready Human Reference Gene Panel in conjunction with the RealTime ready Data Import Wizard from GenEx software provides a very fast and pragmatic approach to identify suitable reference genes, resulting in sound scientific data.

(["Article"], PDF, 2107 KB) Download

RealTime ready RT-qPCR Assay Development and Qualification

In this document, we describe the processes involved in on-demand RealTime ready RT-qPCR assay development for human, mouse, and rat target genes, and provide an overview on key parameters of more than 10,000 RealTime ready RT-qPCR assays.

(["Article"], PDF, 1544 KB) Download

RealTime ready Human Apoptosis Panel, 384

Plate Layout

(["Panel Content and Layout"], PDF, 108 KB) Download

RealTime ready - Custom qPCR Assays and Panels for Human Targets

Gene expression profiling of the human genome on the LightCycler^® 480 System has never been so easy

(["Article"], PDF, 202 KB) Download

RealTime ready Plate Layout Human Cell Cycle Regulation Panel, 96

(["Panel Content and Layout"], PDF, 81 KB) Download

Product Information (1)

Roche Life Science | Single Assays

Use the RealTime ready Custom Single Assays to quantitatively measure gene expression of human, mouse, or rat genes of your choice on real-time PCR Systems.

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