LightCycler® EvoScript RNA SYBR® Green I Master
The LightCycler® EvoScript RNA SYBR® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.
For general laboratory use.
Product No. | Pack Size |
---|---|
07800134001 | 200 x 20 µL reactions |
Product No. | Pack Size |
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07800177001 | 1000 x 20 µL reactions |
To order these products, please contact your Roche order management team.
The LightCycler® EvoScript RNA SYBR® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.
- Ease-of-use, convenience, speed, reduced error
- Result consistency, stability and tolerance of targets of varying secondary structure
- Ability to detect low copy or rare transcripts for gene expression applications
- Superior quantification capability for gene expression applications
- Easier assay optimization
- Contamination control capability
- Generate consistent and robust results, no matter what sample type you are starting from or what target you are detecting
- Rely on a higher sensitivity when detecting low copy numbers, with the input of larger sample volumes into the reaction
- Obtain fast and specific results
Contents
- Master Mix, 5× conc. Contains enzymes for reverse transcription and PCR, RT-qPCR Reaction Buffer, dATP, dCTP, dGTP, and dUTP, Mg(OAc)2, SYBR® Green I dye and proprietary additives
07 800 134 001 2 vials, 440 μL each
07 800 177 001 10 vials, 440 μL each
- Water, PCR grade To adjust the final reaction volume.
07 800 134 001 3 vials, 1 mL each
07 800 177 001 2 vials, 7.25 mL each
The LightCycler® EvoScript RNA SYBR® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.
The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of primers and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR.
In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificity1).
The master mix is optimized for SYBR® Green I applications and does not require optimization with Mg(OAc)2.
1)The reverse transcription activity will be suppressed at temperatures significantly below 60°C.