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LightCycler® EvoScript RNA SYBR® Green I Master

The LightCycler® EvoScript RNA SYBR® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. 

For general laboratory use.

Product No. Pack Size
07800134001 200 x 20 µL reactions
Product No. Pack Size
07800177001 1000 x 20 µL reactions

To order these products, please contact your Roche order management team.

The LightCycler® EvoScript RNA SYBR® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.

 

  • Ease-of-use, convenience, speed, reduced error
  • Result consistency, stability and tolerance of targets of varying secondary structure
  • Ability to detect low copy or rare transcripts for gene expression applications
  • Superior quantification capability for gene expression applications
  • Easier assay optimization
  • Contamination control capability
  • Generate consistent and robust results, no matter what sample type you are starting from or what target you are detecting
  • Rely on a higher sensitivity when detecting low copy numbers, with the input of larger sample volumes into the reaction
  • Obtain fast and specific results


 

Contents

 

  • Master Mix, 5× conc. Contains enzymes for reverse transcription and PCR, RT-qPCR Reaction Buffer, dATP, dCTP, dGTP, and dUTP, Mg(OAc)2, SYBR® Green I dye and proprietary additives

    07 800 134 001   2 vials, 440 μL each
    07 800 177 001  10 vials, 440 μL each

  • Water, PCR grade To adjust the final reaction volume.

    07 800 134 001   3 vials, 1 mL each
    07 800 177 001   2 vials, 7.25 mL each

The LightCycler® EvoScript RNA SYBR® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.
The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of primers and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR.

In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificity1).

The master mix is optimized for SYBR® Green I applications and does not require optimization with Mg(OAc)2.

1)The reverse transcription activity will be suppressed at temperatures significantly below 60°C.