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LightCycler® Uracil-DNA Glycosylase

For general laboratory use. 

Product No. Pack Size
03539806001 100 U (100 reactions)

To order these products, please contact your Roche order management team.

LightCycler® Uracil-DNA Glycosylase hydrolyzes uracil-glycosidic bonds at U-DNA sites in single- and double-stranded DNA, excising uracil and creating alkali- and heat-sensitive abasic sites in the DNA.

  • The enzyme is more active on single-stranded DNA than on double- stranded DNA.
  • The enzyme is inactive on RNA and native, uracil-free DNA.

Contents

The enzyme is supplied in storage buffer at 2 U/µl.

LightCycler® Uracil-DNA Glycosylase (LightCycler® UNG) is ideally suited for preventing carryover contamination between PCRs. This technique involves incorporating deoxyuridine triphosphate (dUTP) into all amplification reactions, and pretreating all successive LightCycler® FastStart Enzyme-based PCR mixtures with LightCycler® UNG. Prior to the thermal-cycling procedure, LightCycler® UNG is added to the LightCycler® amplification reaction mixture, then incubated for 10 minutes at +40°C. During the initial activation of FastStart DNA Polymerase (+95°C for 10 minutes), the LightCycler® UNG becomes inactive.
Note: LightCycler® UNG is designed only for use with the FastStart Enzyme or FastStart Enzyme-based LightCycler® Kits.

Quality

Function test: LightCycler® UNG is function-tested for carryover prevention using the LightCycler® Carousel-Based System.