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LightCycler® 480 High Resolution Melting Master

Ready-to-use hot start reaction mix for real-time PCR and high resolution melting curve analysis using the LightCycler® 480, LightCycler® 96 and LightCycler® Nano Instrument.

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size
04909631001 500 x 20 µL reactions

To order these products, please contact your Roche order management team.

The LightCycler® 480 High Resolution Melting Master is designed for research studies in combination with the LightCycler® 480, LightCycler® 96 and LightCycler® Nano Real-Time PCR System. It is supplied as a ready-to-use 2x-concentrated hot start reaction mix. A separate 25 mM MgCl2 stock solution, supplied with the master, allows users to easily optimize the Mg2+concentration. The LightCycler® 480 High Resolution Melting Master can be used in conjunction with Uracil DNA Glycosylase for carryover prevention during PCR.


  • Achieve consistent, high quality performance with a master mix containing ResoLight, a novel dye that does not inhibit amplification enzymes or interfere with PCR when added during reaction setup.
  • Take advantage of an optimized master mix that is highly stable and robust in handling, ideally suited for use in automated workflows.
  • Save time with a convenient, ready-to-use 2x concentrated hot start master mix. 


1. Master Mix, 2x concentrated, including ResoLight high-resolution melting dye
2. MgCl2 Stock Solution, 25 mM
3. Water, PCR Grade


The LightCycler® 480 High Resolution Melting Master provides reagents, including a hot start PCR enzyme and ResoLight, a novel saturating DNA dye, for amplification and detection of heteroduplex regions in PCR amplicons (e.g., in mutation scanning or genotyping applications). The master mix is optimized for use with the LightCycler® 480, LightCycler® 96 and LightCycler® Nano System and can also be used on other instruments with suitable optics. Data capture can be performed using the detection channel for SYBR Green I assays. The LightCycler® 480 High Resolution Melting Master is compatible with PCR additives (e.g., DMSO) that enhance amplification of GC-rich sequences.


1. Master Mix, 2x concentrated, including ResoLight high-resolution melting dye 
2. MgCl2 Stock Solution, 25 mM 
3. H2O, PCR-grade