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RealTime ready RNA Virus Master

For general laboratory use.

Product No. Pack Size
05619416001 100 x 20 µL reactions
Product No. Pack Size
05992877001 1000 x 20 µL reactions

To order these products, please contact your Roche order management team.

The RealTime ready RNA Virus Master is designed for fast, high sensitive and specific real-time one-step RT-PCR analysis of viral RNA.


  • Highly specific reverse transcription and qPCR detection in a simple one-step "hot start" protocol.
  • Specifically designed for the detection of 10 to 106 copies of viral RNA from a variety of sample materials.
  • Performance is tested and optimized for all LightCycler® Instruments. 


  • Enzyme Blend, 50× concentrated, contains Transcriptor RT, Taq DNA Polymerase and proprietary additives. No activation at +95°C is necessary.
  • Reaction Buffer, 5× concentrated, contains RT-PCR Reaction Buffer, dATP, dCTP, dGTP and dUTP, MgCl2, and proprietary additives
  • Water, PCR Grade, to adjust the final reaction volume 

The RealTime ready RNA Virus Master is designed for fast, high sensitive and specific real-time one-step RT-PCR analysis of viral RNA.
With its 50-fold concentrated mixture of Transcriptor and Taq DNA Polymerase, a unique 5-fold reaction buffer containing dNTPs and MgCl2, the formulation is especially suited for the detection of viral RNA. The proprietary reaction buffer allows a fast and convenient "hot start" RT-PCR without heat pre-activation of the Taq DNA Polymerase. The kit is optimized for HybProbe probes and hydrolysis probes, as well as for Universal ProbeLibrary (UPL) probes, and does not require optimization of MgCl2-concentration.

The RealTime ready RNA Virus Master contains Transcriptor Reverse Transcriptase and a special formulation of Taq DNA Polymerase with proprietary additives that eliminate the need for activation at +95°C.


Each lot of RealTime ready RNA Virus Master is tested to meet specifications of qRT-PCR using in vitro transcribed Hepatitis A Virus (HAV) RNA.