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The LightCycler® Nano Software makes new types of applications possible in just minutes.Sample information, assay settings and analysis results are easily managed.


The LightCycler Nano® Software runs on PCs and Macs.

Quickly and easily use demo files and predefined default settings

For each application, the LightCycler® Nano Software has predefined templates and temperature profile building blocks (e.g., for 2- or 3-step amplification protocols or melting curves), when starting a new run.

Sensitivity settings for each dye and application are easily chosen, and fine-tuned starting from default settings.

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Easily manage sample and target information

Enter sample and target information before, during or after a run. Quickly provide information about individual wells, or a replicate group.

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Analyze fluorescent signals with ease

In a LightCycler® Nano run, all wavelengths between 510 nm and 750 nm are measured simultaneously for all tubes. This means dyes to be used and analyzed do not need to be defined by the user prior to a run.

Simply choose and display the wavelengths you want to analyze at the end of the run, or have a look at the full spectrum.


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Use zoom and lasso functions for data display optimization

Zoom to detect signal nuances (e.g., for differential HRM plots), or to fine tune settings (e.g., to set thresholds for quantification).

Use lasso to easily highlight individual amplification or melting curves.

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Change perspectives easily when analyzing your data

Juxtapose different results, and switch between views. Plot results with respect to either the sample or target.

Conveniently display amplification signals and standard curves next to each other.

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Use intuitive plate views and heat maps

LightCycler® Nano Software plate views match the physical instrument layout, displaying information about each well (e.g., Cq values or genotypes) and enabling easy sample selection.

Color-coded heat maps show the expected Cq values, so you can spot outliers in seconds. Simply place the cursor over a well to display a tooltip with the appropriate Cq value.

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