MagNA Pure LC 2.0 Instrument Software
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One of the most striking features of the MagNA Pure LC 2.0 Instrument is the easy-to-use software, controlling and driving all steps of the nucleic acid isolations as well as the post-elution steps. The Software starts with a title screen and the Main Menu, as shown below. The Main Menu Screen displays the function buttons of the main software functions.
Figure 1: Main Menu Screen of the MagNA Pure LC 2.0 Software.
Before starting a purification run, sample information can be included either by download from a LIMS or a server (filesharing), by scanning a barcode, or by entering them via Touch Screen or keyboard. The user selects the appropriate isolation protocol and provides information on sample parameters:
- Purification protocol
- Sample and elution volumes
- Number of samples
- Name of samples
Sample and protocol parameters are entered on the Sample Ordering Screen. Several protocols can be chosen from a list available in the menu, depending on the nucleic acid to be purified and the sample material (see corresponding Reagent Kits).
Figure 2: Sample Ordering Screen of the MagNA Pure LC 2.0 Software.
On the Stage Setup Screen, the software automatically indicates which reagents and disposables have to be loaded, depending on the number of samples and the protocol chosen.
Figure 3: Stage Setup Screen of the MagNA Pure LC 2.0 Software.
When the purification run is started, the Batch Status Screen displays the time course and the current processing status.
Figure 4: Batch Status Screen of the MagNA Pure LC 2.0 Software.
Once the isolations are completed, the Results Screen is automatically displayed and gives information on the success of the nucleic acid isolations.
Figure 5: Results Screen of the MagNA Pure LC 2.0 Software.
Depending on the user's choice within Sample Ordering, the MagNA Pure LC 2.0 Instrument then automatically proceeds to Post Elution steps. Results can be uploaded to a LIMS or to a server (filesharing).
In addition to its use as a workstation for automated nucleic acid preparation, the MagNA Pure LC 2.0 Instrument can be used as a programmable pipetting robot (e.g., for setup of downstream RT-PCR/PCR) using previously isolated nucleic acids as template. Since this process is performed after nucleic acid elution, it is called Post Elution. During Post Elution steps, the complete setup of PCR is performed as programmed by the user, either directly after a purification run, or separate. The MagNA Pure LC 2.0 Software is fully capable of multi-tasking, meaning that Post Elution steps can be programmed at any time, even during an isolation run. Depending on the PCR platform, the user first has to decide which combination of Cooling Blocks is needed, then programs the pipetting schemes.
Figure 6: Post Elution Edit Screen of the MagNA Pure LC 2.0 Software.
All pipetting steps are controlled by the MagNA Pure LC 2.0 Software. The protocol can be edited using a simple "drag & drop" method and stored for later use. If the PCR reagents have been set up prior to the start of the nucleic acid isolation procedure, the protocol can be run automatically after the isolation has been completed. Since the protocol is stored on the computer's hard drive, it can also be performed and repeated at any other time.
The picture above shows the programming screen for the PCR setup with a LightCycler® Sample Carousel placed in the corresponding Cooling Block. Cooling Blocks are also available to hold Centrifuge Adapters for LightCycler® Capillaries, LightCycler® 480 System or generic 96-well plates, PCR-strips, tubes or A-Rings. In addition, Cooling Blocks containing wells to hold vials pre-loaded with PCR reagents are also available (see the MagNA Pure LC Cooling Blocks product pages for further information).
All pipetting steps are listed in the table of the programming screen. Beyond the setup of PCR mixes, this part of the software can also be used to pool several isolations or to prepare a dilution series of the isolated nucleic acids. Once programming is finished, the pipetting process can be started or saved for later use. All steps will be performed by the MagNA Pure LC 2.0 Instrument exactly as programmed. The end of this process is shown by the Post-Elution Results Screen (see below). The isolated nucleic acids are now ready to be analyzed by PCR. The results can be uploaded to a LIMS or on a server (filesharing).
Figure 7: Post-Elution Results Screen of the MagNA Pure LC 2.0 Software.