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Roche Oncology Life Science Research Kits

Our answers. Your insights.

The Roche Oncology Life Science Research (LSR) kits put our research to work in your lab. These kits comprehensively deliver what you need to make your next discovery – starting with more information. The tests come with access to a free1, real-time data analysis portal for automated, objective interpretation of results, while still giving you the raw data that can help in developing important insights. All this, plus the unparalleled Roche support you can count on.

It’s more than a new LSR portfolio. It’s the answer you’ve been looking for.

The video introduces the Roche Diagnostics’ Oncology Life Science Research Kits and explains the workflow of the LSR assays – starting with the BRAF/NRAS Mutation Test (LSR). It reviews the test features and attributes, highlights the rapid turnaround time, and walks through the simple results reporting step-by-step.

1 free for registered Roche life science users

Helpful Links

Roche Oncology Life Science Research Kits FAQ

Roche Oncology LSRs are complete testing kits that are designed to fuel your research. With comprehensive coverage of oncology mutations and a web application to analyze your data, the Oncology LSRs provide the information you need to garner real insights for oncology research.

No. When you use the Web Portal, results are delivered direct to you. Roche does not store, view, or use your data.

Interpreted data is available for 24 hours. After this period, data will need to be re-uploaded for reinterpretation.

Once you upload the raw data into the online Data Analysis tool, the results are delivered to you within a few minutes.

Turn on the cobas z 480 analyzer if it is turned off. If necessary, reboot the cobas® 4800 workstation and log into the User defined Workflow (UDF) partition on the system. Once the system has booted up, follow the appropriate assay Instructions for Use for more information on the Run Setup.

Roche recommends the cobas® DNA Sample Preparation Kit, however, the Roche Oncology LSRs are robust enough to work with a variety of DNA sample preparation methods. Per the Instructions for Use, the test requires 150ng of genomic DNA. Please see the appropriate assay Instructions for Use for more detailed information regarding DNA preparation.

The Roche Oncology LSRs come with the same quality of support you get with other Roche assays. Roche representatives are available for troubleshooting and training as needed. For specific questions and concerns, please contact your local Roche representative.

The Data Analysis tool is best viewed in Google Chrome 3 or above, Mozilla Firefox 3.5 or above, and Microsoft Internet Explorer 7 or above.

These products are currently not available in the United States.


Data Analysis

 

The LSR Data Analysis tool is a free1 service that gives researchers access to Roche Diagnostics' unique testing algorithms to automatically interpret data generated by the Oncology LSR test kits. Rather than having to analyze all of the individual data points manually, researchers can have data files analyzed and results generated in .pdf and .csv documents within minutes.

 

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Helpful Links

 

1 free for registered Roche life science users

These products are currently not available in the United States.

BRAF/NRAS Mutation Test (LSR)

The BRAF/NRAS Mutation Test (LSR) from Roche is an allele-specific, real-time PCR test for the qualitative detection and identification of exon 11 and 15 mutations in the protooncogene B-Raf (BRAF) gene and exon 2, 3, and 4 mutations in the neuroblastoma RAS (NRAS) viral oncogenes from FFPET or plasma samples.

BRAF/NRAS Mutation Test (LSR)

One test kit for use with FFPET and plasma samples.

Key Features and Benefits

What are Roche Oncology Life Science Research (LSR) kits?

Possibility of broad detection of BRAF and NRAS mutations within an 8-hour laboratory shift

  • Results from a single, 5µm FFPET section of tissue or 2mL plasma
  • Liquid, ready-to-use reagents to increase laboratory efficiency
  • A 12-month shelf life from date of manufacture1
  • A free2, web-based analysis tool enables rapid, automated, and objective interpretation of results
  • 100% repeatability over multiple specimens, operators, instruments, and days1
  • Sensitivity of 5% or greater mutant alleles in a wild-type background in DNA isolated from FFPET samples1
  • Sensitivity as low as 100 copies/mL in DNA isolated from plasma samples

 

1. BRAF/NRAS LSR Package Insert
2. The LSR Data Analysis Portal is free for all registered users of Roche at lifescience.roche.com

Roche Oncology

BRAF/NRAS LSR TESTING WORKFLOW

When you’re on the path to discovery, every result matters. Every answer could be the one that gets you there. To the revelation you were searching for. To the advancement you needed. To your next breakthrough. The Roche Life Science Research Assay Portfolio offers the speed, accuracy, and open-source flexibility your research demands. So you get clear, actionable results faster, in fewer shifts, and backed by the exacting standards, quality, and service of Roche.

COVERAGE OF THE BRAF/NRAS MUTATION TEST (LSR):
NRAS COSMIC v.80   SKIN COLON
Freq of Reports LSR Coverage Freq of Reports LSR Coverage
exon 2 13.0% 11.8% 42.9% 42.4%
exon 3 86.6% 84.4% 55.8% 54.7%
exon 4 <1%
<1% <1% <1%
TOTAL 99.5% 96.3% 98.7% 97.1%
BRAF COSMIC v.80   SKIN COLON
Freq of Reports LSR Coverage Freq of Reports LSR Coverage
exon 11 <1% <1% <1% <1%
exon 15 98.7% 96.3% 99.4% 98.4%
TOTAL 99.0% 96.5% 99.6% 98.5%
PRODUCT DESCRIPTION QUANTITY MATERIAL NUMBER
BRAF/NRAS Mutation Test (LSR) 24 07659962001
cobas® 4800 UDF v2.0.0 CD 1 07540922001
cobas® 4800 Microwell Plate and Sealing Foil 50 05232724001

FOR MORE INFORMATION

Contact your local Roche Molecular Diagnostic representative or visit molecular.roche.com

For printable BRAF/NRAS Mutation Test (LSR) brochure, click here.

These products are currently not available in the United States.

KRAS Mutation Test v2 (LSR)

The KRAS Mutation Test v2 (LSR) from Roche is an allele-specific, real-time PCR test for the qualitative detection and identification of 28 mutations in exons 2, 3, and 4 of the v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) gene from FFPET and plasma samples.

KRAS Mutation Test v2 (LSR)

An allele-specific, real-time PCR test for the detection of 28 unique KRAS mutations in codons 12, 13, 59, 61, 117, and 146

Key Features and Benefits

What are Roche Oncology Life Science Research (LSR) kits?

Possibility of broad detection of KRAS mutations within an 8-hour laboratory shift

  • Results from a single, 5µm FFPET section of tissue or 2mL plasma
  • Liquid, ready-to-use reagents to increase laboratory efficiency
  • 18-month shelf life from date of manufacture1
  • A free2, web-based analysis tool enables automated, objective, and rapid interpretation of results
  • 100% repeatability over multiple specimens, operators, instruments and days1
  • Validated in FFPET samples and can reliably detect KRAS mutations in exons 2, 3 and 4 mutations at a percent mutation of 1% or greater1
  • Validated in plasma samples with sensitivity of 75 copies/mL or lower in a wild-type background of 64,000 copies1

 

1. KRAS v2 LSR Package Insert
2. The LSR Data Analysis Portal is free for all registered users of Roche at lifescience.roche.com

Roche Oncology
KRASv2LSR Testing Workflow

KRAS v2 LSR TESTING WORKFLOW

When you’re on the path to discovery, every result matters. Every answer could be the one that gets you there. To the revelation you were searching for. To the advancement you needed. To your next breakthrough. The Roche Life Science Research Assay Portfolio offers the speed, accuracy, and open-source flexibility your research demands. So you get clear, actionable results faster, in fewer shifts, and backed by the exacting standards, quality, and service of Roche.

COVERAGE OF THE KRAS MUTATION TEST v2 (LSR):
KRAS COSMIC v.81   COLON LUNG PANCREAS
Freq of Reports LSR Coverage Freq of Reports LSR Coverage Freq of Reports LSR Coverage
exon 2 98.3% 97.7% 98.7% 97.7% 98.8% 98.3%
exon 3 1.0% 0.9% 1.2% 1.1% 1.1% 1.0%
exon 4 0.7% 0.5% 0.06% 0.04% 0.08% 0.04%
all others* 0.01% 0.0% 0.04% 0.0% 0.0% 0.0%
TOTAL 100.0% 99.1% 100.0% 98.8% 100.0% 99.3%

* mutations outside exon 2, 3 and 4.

PRODUCT DESCRIPTION QUANTITY MATERIAL NUMBER
KRAS Mutation Test v2 (LSR) 24 07989270001
cobas® 4800 UDF v2.0.0 CD 1 07540922001
cobas® 4800 Microwell Plate and Sealing Foil 50 05232724001

FOR MORE INFORMATION

Contact your local Roche Molecular Diagnostic representative or visit molecular.roche.com

For printable KRAS Mutation Test v2 (LSR) brochure, click here

These products are currently not available in the United States.