MagNA Pure 96 Instrument
High-throughput robotic workstation for fully automated purification of nucleic acids from up to 96 samples.
For in vitro diagnostic use.
Product No. | Pack Size |
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06541089001 | 1 instrument, control unit and accessories |
To order these products, please contact your Roche order management team.
The MagNA Pure 96 System purifies DNA, RNA, and viral nucleic acids from a wide range of starting materials using magnetic glass particle technology.
Preprogrammed software protocols are tailored to a diversity of sample requirements. Resulting nucleic acids are suitable for a broad range of downstream applications.
The MagNA Pure 96 System effortlessly processes up to 96 samples in less than one hour using barcoded, prefilled trays with ready-to-use reagents.
Experience seamless data management using intuitive features, such as the link to a laboratory information management system (LIMS).
Feature: |
Benefit: |
Speed and throughput
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Quick time-to-result for clinicians and patients |
Convenience
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Minimized error risk |
Robustness
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Confidence & trust in your results |
Performance
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One solution for various needs |
Service and support
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Minimum downtime |
The MagNA Pure 96 System is a high-throughput instrument for automated nucleic acid purification. Typical run duration is less than one hour for 96 standard volume samples. Applications are very flexible, due to a diversity of software purification protocols using barcoded, prefilled trays with ready-to-use reagents.
MagNA Pure 96 kits are designed profiting from our more than ten year expertise in magnetic glass particle technology. We have streamlined the purification process to a very small number of reagents, consumables and steps.
Optimized preprogrammed purification protocols ensure routine sample handling and high quality, reproducible nucleic acid yields. Choose the appropriate purification protocol for your sample material and target nucleic acid:
Target: Bacterial, fungal and viral NA | |||||||||||||||
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Protocol name | Input sample type and sample volumes | Elution volume | Run time** (h:mm) |
||||||||||||
Whole blood | Plasma (citrate) | Plasma (EDTA) | Serum | Swabs | BAL* | Sputum | CSF | Urine | Stool | Cultured cells | Fresh-frozen tissue | FFPET | |||
Pathogen Universal 2001 | 200 μL | 200 μL | 200 μL | 200 μL | 200 μL | 200 μL | 200 μL | 200 μL | 200 μL | 200 μL | 50, 100 μL |
1:00 | |||
Viral NA Plasma SV1 | 50, 100, 200 μL |
50, 100 μL |
1:00 | ||||||||||||
Viral NA Plasma ext lys SV1 Protocol optimized with external lysis reagent |
100, 200 μL | 100, 200 μL | 100, 200 μL | 100, 200 μL | 50, 100 μL |
1:00 | |||||||||
Viral NA Universal SV1 | 50, 100, 200 μL | 50, 100, 200 μL | 50, 100, 200 μL | 50, 100 μL |
1:00 | ||||||||||
Pathogen Universal 5002 |
500 μL |
500 μL |
500 μL | 500μL | 500 μL | 500 μL | 500 μL | 500 μL | 500 μL | 500 μL | 50, 100 μL |
1:30 | |||
Pathogen Universal 10002 |
1000 μL | 1000 μL | 1000 μL | 1000 μL | 1000 μL | 1000 μL | 1000 μL | 1000 μL | 1000 μL | 1000 μL | 50, 100 μL |
1:40 | |||
Viral NA Plasma LV2 | 500 μL |
50, 100 μL |
1:10 | ||||||||||||
Viral NA Plasma LV 10002 | 1000 μL |
50, 100 μL |
1:20 | ||||||||||||
Viral NA Plasma ext lys LV2 |
500 μL |
500 μL |
500 μL |
50, 100 μL |
1:10 | ||||||||||
Viral NA Universal LV2 | 500 μL |
500 μL |
50, 100 μL |
1:30 | |||||||||||
Viral NA Universal LV 10002 | 1000 μL |
1000 μL |
50, 100 μL |
1:30 | |||||||||||
1 MagNA Pure 96 DNA and Viral NA Small Volume Kit (cat. no. 06 543 588 001) |
*BAL - Bronchoalveolar Lavage |
||||||||||||||
To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), and MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001). Please refer to the Instructions for Use manual for more details. |
Target: Genomic DNA | |||||||||||||||
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Protocol name | Input sample type and sample volumes | Elution volume | Run time** (h:mm) |
||||||||||||
Whole blood | Plasma (citrate) | Plasma (EDTA) | Serum | Swabs | BAL* | Sputum | CSF | Urine | Stool | Cultured cells | Fresh-frozen tissue | FFPET | |||
DNA Blood SV1 | 50, 100, 200 μL |
50, 100 μL |
1:00 | ||||||||||||
DNA Blood ext lys SV1 Protocol optimized with external lysis reagent |
50, 100, 200 μL |
50, 100, 200 μL |
50, 100, 200 μL |
50, 100, 200 μL |
50, 100 μL |
0:50 | |||||||||
DNA Cells SV1 | Up to 5x105 cultured cells in 200 μL PBS | 100 μL | 1:00 | ||||||||||||
DNA Tissue SV1 | Up to 5mg in 200 μL | 100, 200 μL | 1:20 | ||||||||||||
DNA FFPE SV1 | 1x5-10 um | 50, 100 μL | 1:00 | ||||||||||||
DNA Blood LV2 | 500 μL | 100, 200 μL |
1:20 | ||||||||||||
DNA Blood LV 10002 | 1000 μL | 100, 200 μL |
1:30 | ||||||||||||
DNA Blood ext lys LV2 Protocol optimized with external lysis reagent |
500 μL |
500 μL |
500 μL |
500 μL |
100, 200 μL |
0:50 | |||||||||
Up to 1x106 cultured cells in 200 μL PBS | 100, 200 μL |
1:20 | |||||||||||||
DNA Tissue LV2 | Up to 25 mg in 200 μL |
200 μL | 1:20 | ||||||||||||
1 MagNA Pure 96 DNA and Viral NA Small Volume Kit (cat. no. 06 543 588 001) |
*BAL - Bronchoalveolar Lavage **These times are approximate and should only be used as a guidance. |
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To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001), and MagNA Pure FFPET Buffer Set (Cat. No. 08 447 144 001). Please refer to the Instructions for Use manual for more details. |
Target: Cell-free NA | |||||||||||||||
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Protocol name | Input sample type and sample volumes | Elution volume | Run time** (h:mm) |
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Whole blood | Plasma (citrate) | Plasma (EDTA) | Serum | Swabs | BAL* | Sputum | CSF | Urine | Stool | Cultured cells | Fresh-frozen tissue | FFPET | |||
cfNA ss 20002 Protocol optimized for single-stranded DNA |
2000 μL |
50, 100 μL |
2:00 | ||||||||||||
cfNA ss 40002 Protocol optimized for single-stranded DNA |
4000 μL |
50, 100 μL |
2:20 | ||||||||||||
cfNA ds 20002 Protocol optimized for double-stranded DNA |
2000 μL |
100, 200 μL |
2:20 | ||||||||||||
cfNA ds 40002 Protocol optimized for double-stranded DNA |
4000 μL |
100, 200 μL |
2:50 | ||||||||||||
2 MagNA Pure 96 DNA and Viral NA Large Volume Kit (cat. No. 06 374 891 001) | *BAL - Bronchoalveolar Lavage |
||||||||||||||
To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001), and MagNA Pure FFPET Buffer Set (Cat. No. 08 447 144 001). Please refer to the Instructions for Use manual for more details. |
Target: Total RNA | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Protocol name | Input sample type and sample volumes | Elution volume | Run time** (h:mm) |
||||||||||||
Whole blood | Plasma (citrate) | Plasma (EDTA) | Serum | Swabs | BAL* | Sputum | CSF | Urine | Stool | Cultured cells | Fresh-frozen tissue | FFPET | |||
Cellular RNA LV3 |
Up to 1x106 cultured cells in 200 μL PBS | 50, 100, 200 μL | 1:30 | ||||||||||||
RNA Blood LV 4003 | 400 μL | 50, 100, 200 μL | 1:30 | ||||||||||||
RNA Blood LV 8003 | 800 μL | 100, 200 μL |
1:30 | ||||||||||||
RNA PAXgene LV3 | PAXgene pellet in 400 μL PBS | 100, 200 μL |
1:30 | ||||||||||||
RNA PAXgene Half Tube LV3 | Half PAXgene pellet in 200 μL PBS | 50, 100 μL |
1:30 | ||||||||||||
RNA Tissue FF Standard LV3 | Up to 10 mg in 350 μL | 50, 100, 200 μL | 1:30 | ||||||||||||
Up to 25 mg in 700 μL | 100, 200 μL | 1:30 | |||||||||||||
1x 5-10μm section | 50, 100 μL |
1:30 | |||||||||||||
3 MagNA Pure 96 Cellular RNA Large Volume Kit (cat no. 05 467 535 001) | *BAL - Bronchoalveolar Lavage |
||||||||||||||
To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001), and MagNA Pure FFPET Buffer Set (Cat. No. 08 447 144 001). Please refer to the Instructions for Use manual for more details. |
Target: Liquid handling | |||||||||||||||
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Protocol name | Input sample type and sample volumes | Elution volume | Run time** (h:mm) |
||||||||||||
Whole blood | Plasma (citrate) | Plasma (EDTA) | Serum | Swabs | BAL* | Sputum | CSF | Urine | Stool | Cultured cells | Fresh-frozen tissue | FFPET | |||
Sample Transfer to the processing cartridge |
N/A | ||||||||||||||
Archiving Plate Transfer for archiving purposes |
5 μL to 200 μL | N/A | |||||||||||||
PCR Setup with Mixing | 5 μL to 200 μL |
N/A | |||||||||||||
PCR Setup without Mixing | 5 μL to 200 μL |
N/A | |||||||||||||
*BAL=Bronchoalveolar Lavage |
|||||||||||||||
To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), and MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001), and , and MagNA Pure FFPET Buffer Set (Cat. No. 08 447 144 001). Please refer to the Instructions for Use manual for more details. |
Discover effortless nucleic acid isolation
Magnetism is used for automated nucleic acid isolation performed by the MagNA Pure 96 System from Roche. The high throughput MagNA Pure 96 Instrument performs automated nucleic acid purification from samples up to 4 mL, pipetted into a 96-well processing cartridge.
Magnetic glass particles (MGPs) are used to isolate RNA, DNA and total nucleic acids (tNA) from a variety of different sample materials. The MagNA Pure 96 System permits automated nucleic acid purification that matches the high speed, accuracy and reliability of LightCycler® Real-Time PCR Systems.
Key features of the MagNA Pure 96 System are:
Sample Number: |
1 to 96 reactions per run |
Sample volume: |
|
Maximum number of sample types within one run: |
10 (whole blood, plasma, serum, urine, swabs, stool, sputum, BAL, CSF, bacterial cultures) |
Run time for 96 reactions: |
|
Reagent type: |
Prefilled, ready-to-use |
LIMS connectivity: |
Yes |
21CFR part 11 (subsection B) compatibility: |
Yes |
IVD compliance: |
Instrument complies with the requirements of IVD directive 98/79/EC.
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The Magna Pure 96 system is on software version 3.1 currently.
If you need assistance to update your system software, please contact your local Roche representative.