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LifeScience
High Pure FFPET RNA Isolation Kit

High Pure FFPET RNA Isolation Kit

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size
06650775001 50 purifications
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To order these products, please contact your Roche order management team.

Keywords: Manual Isolation & Purification, High Pure, Nucleic Acid Type, RNA

The High Pure FFPET RNA Isolation Kit is designed for the isolation and purification of total RNA from 5 to 10 μm thick sections from formalin-fixed, paraffin-embedded tissue. Macrodissected samples or biopsy material can also be used. Isolated RNA is suitable for qRT-PCR, and microarray analysis.

 

  • Total time required is approximately 2.5 hours including deparaffinization procedure.
  • RNA recovery in 25 to 50 μL elution volume
  • Obtain high yields of contamination-free RNA 
     

Contents

  • Tissue Lysis Buffer
  • Proteinase K, PCR-grade 
  • RNA Binding Buffer
  • Wash Buffer I 
  • Wash Buffer II
  • DNase I
  • DNase Incubation Buffer
  • Elution Buffer
  • Reagent Preparation Buffer
  • High Pure Filter Tubes  
  • Collection Tubes

The deparaffinized  tissue is lysed with a special lysis buffers and incubated with Proteinase K. In the presence of chaotropic salts, the RNA is specifically bound to the glass fiber of the High Pure Filter Tube. Bound RNA is incubated with DNAse and purified in a series of rapid wash-and- spin steps and then eluated from the column.

Associated Products

LightCycler® EvoScript RNA SYBR® Green I Master Read more
LightCycler® EvoScript RNA Probes Master Read more