LightCycler^® 1536 System Performance Data

Read in this article:

• Sensitivity and Reproducibility
  • Superb Linear Range
    Outstanding Sensitivity and Reproducibility
• Real-Time PCR Results
  • Precision Melting Curve Analysis for DNA Product Identification
    Sensitive Detection of Endpoint Fluorescence (EPF) Values
• Application Data
  • High-Throughput Gene Expression Profiling
    Long-Term Stability

Figure 1: Superb linear range of the LightCycler^® 1536 System. Eight concentration steps of a 1:10 serial dilution from 10⁷ to 10⁰ copies/sample and negative controls (96 replicates each).

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Figure 2: Outstanding sensitivity and reproducibility of the LightCycler^® 1536 System. Sixteen concentration steps of a 1:2 serial dilution from 10⁶ to 3 x 10¹ copies/sample (96 replicates each) distributed in a checkerboard scheme across the entire multiwell plate, as depicted in the crossing point heatmap chart (6-times 4 x 4 replicates).

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Figure 3: Precision melting curve analysis for DNA product identification with the LightCycler^® 1536 System. Melting peak curves of two individual target genes, illustrated as a first negative derivative plot of the fluorescence versus temperature.

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Figure 4: Sensitive detection of endpoint fluorescence (EPF) values. Amplification curves and one corresponding heatmap showing endpoint values of a dual-color fluorescence detection analysis.

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Figure 5: High-Throughput Gene Expression Profiling.

Experimental setup:

Sixteen tissue samples were analyzed using 96 RealTime ready Assays, with 82 target genes from the apoptosis pathway, 8 reference genes, and 6 controls.

Results:

• LightCycler^® 1536 Software:
  a) Amplification data for all 1536 real-time PCR assays; b) Single analysis of 1 target gene (AKT1) in 16 tissue samples; c) Single analysis of 82 target genes in 1 tissue sample (small intestine)
• Downstream software:
  connectionParserd) Normalized ratios of 1 target gene (AKT1) in 15 tissue samples; e) Normalized ratios of 82 target genes in 1 tissue sample (small intestine)

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