EvoScript RNA SYBR® Green I Master
The EvoScript RNA SYBR® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.
For general laboratory use.
| Product No. | Pack Size |
|---|---|
| 07912480001 | 200 reactions |
To order these products, please contact your Roche order management team.
The EvoScript RNA SYBR® Green I Master as a 1-vial composition, is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays
Benefits
- Ease-of-use, convenience, speed, reduced error
- Result consistency, stability and tolerance of targets of varying secondary structure
- Ability to detect low copy or rare transcripts for gene expression applications
- Superior quantification capability for gene expression applications
- Easier assay optimization
- Contamination control capability
- Generate consistent and robust results, no matter what sample type you are starting from or what target you are detecting
- Rely on a higher sensitivity when detecting low copy numbers, with the input of larger sample volumes into the reaction
- Obtain fast and specific results
Contents
1. EvoScript RNA SYBR® Green I Master, 5× conc.
2. Water, PCR Grade
The EvoScript RNA SYBR® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of primers and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR. In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificity1). The master mix is optimized for SYBR® Green I applications and does not require optimization with Mg(OAc)2.
1) The reverse transcription activity will be suppressed at temperatures significantly below 60°C.
Instructions for Use (IFU)
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