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Publications (17)

Identification of ryanodine receptor 1 single-nucleotide polymorphisms by high-resolution melting using the LightCycler 480 System 

Keywords: Lightcycler 480 ,LightCycler probe design software

Hilbert G. and Kathryn S.

Anal Biochem, 374, 2, 396 - 404

Methylation-sensitive high-resolution melting 

Keywords: Lightcycler 480 ,Lightcycler 480 ResoLight Dye ,Taq polymerase ,Light Cycler 480 HRM Master ,Resolight Dye

T. Wojdacz , A. Dobrovic and L. Hansen

Nature Protocols, 3, 1903 - 1908

Detection of High-Frequency and Novel DNMT3A Mutations in Acute Myeloid Leukemia by High-Resolution Melting Curve Analysis 

Keywords: LightCycler480 ,Genotyping ,HRM ,Cancer Research

R. R. Singh , A. Bains , K. P. Patel , H. Rahimi , B. A. Barkoh , A. Paladugu , T. Bisrat , F. Ravandi-Kashan , J. E. Cortes , H. M. Kantarjian , L. .J.Medeiross , R. Luthra

The Journal of Molecular Diagnostics, 14, 4, Pages 336-345

A rapid high-throughput method for the detection and quantification of RNA editing based on high-resolution melting of amplicons 

Keywords: Lightcycler 480 ,Lightcycler 480 Probes Master Mix

A. Chateigner-Boutin and I. Small

Nucleic Acids Res, 35, 17, e114 -

Use of MagNA Pure 96 and LightCycler® Systems for High Throughput Testing of Nail Samples for Dermatophytes Causing Onychomycosis

 

Keywords: MagNA Pure 96 ,Lightcycler 480 ,Nail samples

E. Snelders , A. Jong , P. Verwij and W. Melchers

Application Note 7

Documents (5)

Transfering PCRs to HRM-assays on the LightCycler® 480 System - Examples for BRCA1

To evaluate how easy it is to design hrMCA assays using the LightCycler® 480 Instrument, we selected 3 different gene fragments, designed an MCA assay, and tested its sensitivity to detect known variants.

(["Article"], PDF, 248 KB) Download

LightCycler® 480 Real-Time PCR System - High Resolution Melting: Optimization Strategies

High resolution melting (HRM) is a novel, closed-tube, post-PCR technique allowing genomic researchers to easily analyze genetic variations in PCR amplicons.

(["Article"], PDF, 3386 KB) Download

Rapid High-Throughput Methylation Analysis Using the LightCycler® 480 System

We assessed the performance of the instrument combined with the LightCycler® 480 High Resolution Melting Master mix for DNA methylation analysis using the methylation-sensitive high resolution melting (MS-HRM) methodology.

(["Article"], PDF, 177 KB) Download

High Resolution Melting: Optimization Strategies

This technical note describes general steps of setting up HRM-based PCR assays, with a special focus on ways how to optimize procedures for gene scanning experiments

(["Article"], PDF, 3386 KB) Download

Rapid and Reliable Methylation Detection in Archival Tissue Samples using High-Resolution Melting Analysis

The aim of the present study was to establish and evaluate HRM assays for detection of promoter methylation on archival FFPE tissues from individuals with colorectal cancer.

(["Article"], PDF, 986 KB) Download
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