RealTime ready Pre-Amp Primer Pool
For life science research only. Not for use in diagnostic procedures.
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To order these products, please contact your Roche order management team.
- Select and order a custom primer pool for your RealTime ready Custom Panel.
- High quality primer pairs, ensure a virtually bias-free target specific pre-amplification.
- Ideally suited for pre-amplification of cDNA derived from RNA isolated from formalin-fixed, paraffin-embedded tissue samples.
RealTime ready cDNA Pre-Amp Primer Pool, 4× concentrated
The RealTime ready Pre-Amp Primer Pool contains a mixture of primers from custom selected RealTime ready Assays in a ready-to-use concentration for cDNA multiplex cDNA pre-amplification PCR. It is used with the RealTime ready cDNA Pre-Amp Master. Pre-amplified cDNA is then applied to the corresponding RealTime ready Custom Panel. The primer pool is customized by configuring a RealTime ready Panel on the online portal of the RealTime ready Configurator.
Note: RealTime ready Pre-Amp Primer Pool containing Designer Assays must be ordered along with the corresponding RealTime ready Custom Panel.
cDNA is pre-amplified in a multiplex PCR using custom RealTime ready Pre-Amp Primer Pools containing 6 to 96 specific primer pairs corresponding to the primers that you require for RealTime ready Assays you have selected.
The resulting product is a uniform doublestranded pre-amplification of your selected targets, without the bias that can be produced using whole transcriptome amplification technologies. This pre-amplified cDNA is ideal for use with pre-plated, function tested RealTime ready Assays.