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LifeScience
LightCycler<sup>®</sup> Multiplex DNA Master

LightCycler® Multiplex DNA Master

Easy-to-use 5x reaction mix optimized for multiplex qPCR, compatible with the LightCycler® 480 or the LightCycler® 96 Real-Time PCR Systems.

For general laboratory use.

Product No. Pack Size
07339577001 1000 x 20 µL reactions
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Product No. Pack Size
07339585001 200 x 20 µL reactions

To order these products, please contact your Roche order management team.

Keywords: qPCR Master Mixes, Pathogen Detection

Rely on this new Real-Time PCR Master Mix for fast and highly sensitive multiplex detection of DNA targets and internal controls. Based on the aptamer-mediated hot start AptaTaq Fast DNA Polymerase and a 5x concentrated buffer formulation optimized for multiplex target detection, enabling sensitive qPCR of pathogen DNA targets isolated from a variety of human sample materials.
 

  • Perform fast, highly sensitive and specific qPCR detection of pathogen DNA targets isolated from a wide range of materials, including difficult sample materials (e.g., blood, plasma, serum, stool)
  • Increase throughput and cost efficiency of the qPCR workflow with a master mix optimized for multiplex detection of up to 5 targets
  • Detect low copy number of DNA targets with higher sensitivity with a 5x master mix concentration
  • Decrease experiment set up time and effort with convenient single vial mix
  • Automate your workflow or setup your reactions at room temperature with the thermostable enzyme in the master mix

 

Contents

 

  • qPCR Reaction Mix, 5x conc.
  • Water, PCR grade

The LightCycler® Multiplex DNA Master is a 5x master mix especially suited for the fast and efficient multiplex PCR detection of up to 5 DNA pathogen targets in human sample materials. The robust formulation allows multiplex qPCR detection of a multitude of pathogen targets without the need for reaction condition adaptation or titration of MgCl2 concentration. The PCR protocol has been designed to run on the LightCycler® 480 and LightCycler® 96 Systems. The master mix is optimized for use with hydrolysis probes as well as Universal ProbeLibrary (UPL) probes.


The LightCycler® Multiplex DNA Master consists of a complete 5x qPCR reaction mix with AptaTaq Fast DNA Polymerase, dNTPs, and a special reaction mix for multiplex qPCR. AptaTaq Fast DNA Polymerase is a thermostable, recombinant Taq polymerase with aptamer-mediated, reversible hot start. It is immediately activated by heating, which allows specific priming and fast PCR. The working solution is stable at +15 to +25°C up to 4 hours and is therefore ideal for use in automated workflows.

Associated Products

High Pure FFPET DNA Isolation Kit Read more
DNA Process Control Kit Read more
RNA Process Control Kit Read more
LightCycler® 480 Multiwell Plate 96, white Read more
High Pure Viral Nucleic Acid Kit Read more
High Pure PCR Template Preparation Kit Read more