MagNA Pure Compact Nucleic Acid Isolation Kit I
Ready-to-use hands-free reagents in prefilled, sealed cartridges for purifying DNA from mammalian cells and total viral nucleic acids in a final volume of up to 400 µL with the MagNA Pure Compact Instrument.
For general laboratory use.
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- Isolate genomic DNA from mammalian whole blood, blood cells, buffy coat, or cultured cells.
- Isolate viral nucleic acids from serum or plasma.
- Isolate bacterial DNA from different sample types.
- Use for sample volumes of 100 to 400 µl.
- Reagent Cartridges, 32 sealed cartridges
- Tip Tray, 32 disposable Tip Trays
- MagNA Pure Tubes, 2.0 mL
- MagNA Pure Elution Tube Caps
For general laboratory use.
The MagNA Pure Compact Nucleic Acid Isolation Kit I is specifically designed to isolate highly purified genomic DNA from mammalian whole blood, blood cells, buffy coat, or cultured cells, and total nucleic acids (e.g., viral DNA and RNA) from mammalian serum or plasma, using the MagNA Pure Compact Instrument.In combination with the MagNA Pure Bacteria Lysis Buffer and the DNA_Bacteria purification protocol, bacterial DNA can be isolated from different types of sample of mammalian origin (such as urine, BAL, sputum, CSF, swabs) or bacterial cultures. The purified nucleic acids can be used in PCR or RT-PCR on the LightCycler® Carousel-Based Systems, theLightCycler® 480 System, or standard thermal block cyclers.
Number of isolations/sample type: 32 isolations (4 × 8) from
- 100 to 400 μl mammalian whole blood (containing no more than 5 × 103 cells/μl)
- 100 to 400 μl suspension of mammalian blood cells (white blood cells [WBCs] or peripheral blood mononuclear cells [PBMCs] or buffy coat [containing no more than 5 × 103 cells/μl])
- 100 to 400 μl mammalian serum or plasma
- 100 μl suspension of cultured cells (containing no more than 1 × 106 cells)
If combined with MagNA Pure Bacteria Lysis Buffer 200 μl of liquified samples of mammalian origin ( e.g., stool, urine, bronchoalveolar lavage [BAL], sputum, cerebrospinal fluid [CSF], swabs or bacterial cultures) For details, see - Instructions for Use
To isolate genomic DNA or total nucleic acid from larger-volume samples (500 to 1,000 μl), choose the MagNA Pure Compact Nucleic Acid Isolation Kit I – Large Volume
To isolate DNA in combination with external lysis, the DNA_Blood_external_lysis or the Total_NA_Plasma_external_lysis protocol is required. To isolate bacterial DNA with the MagNA Pure Compact Instrument, the DNA_Bacteria purification protocol is required. If the required protocol is not installed (and listed in the protocol selection field) in the MagNA Pure Compact Software, download the protocol.
- Binnicker, M.J. et al. (2007). Detection of Coccidioides Species in Clinical Specimens by Real-Time PCR.. J. Clin. Microbiol ., 45: 173 - 178.
- Koidl, C. et al. (2007). Detection and Differentiation of Bordetella spp. by Real-Time PCR. J. Clin. Microbiol ., 45: 347 - 350.
- Lin, B. et al. (2007). Using a Resequencing Microarray as a Multiple Respiratory Pathogen Detection Assay. J. Clin. Microbiol ., 45: 443 - 452.
- Baumgartner et al. (2005). MagNA Pure Compact System: Purification of High-Quality DNA from PBMCs, WBCs, and Buffy Coat. Biochemica 2: 9-11.
- Reischl et al. (2005). Automated Rapid Isolation of Bacterial DNA from Various Samples Using the MagNA Pure Compact System. Biochemica 2: 12-15.
- Uhl et al. (2005). Use of the Roche LightCycler Strep B Assay for Detection of Group B Streptococcus from Vaginal and Rectal Swabs. J. Clin. Microbiol ., 43: 4046-4051.
- Waltenberger et al. (2004). MagNA Pure Compact System – Results of a Performance Study for Viral Nucleic Acid Isolation from Serum and Plasma Samples. Biochemica 3: 10-11.
- Kirchgesser et al. (2003). The New MagNA Pure Compact Nucleic Acid Isolation Kits – Fast and Flexible Fully Automated Sample Preparation. Biochemica 4: 12-15.
The nucleic acid isolation procedure is based on the proven MagNA Pure Magnetic Glass Particle Technology.
The principle steps of a MagNA Pure Compact nucleic acid isolation procedure are:
- The samples are lysed by incubation with Proteinase K and a special lysis buffer containing a chaotropic salt.
- Magnetic Glass Particles (MGPs) are added and nucleic acids are immobilized on the MGPs surfaces.
- Unbound substances (e.g., proteins, cell debris, PCR inhibitors etc) are removed by several washing steps.
- Purified nucleic acids are eluted from the MGPs.