MagNA Pure LC 2.0 Instrument
For general laboratory use.
|Product No.||Pack Size|
|05197686001||1 instrument, integrated PC, touchscreen monitor, and accessories|
To order these products, please contact your Roche order management team.
The MagNA Pure LC 2.0 Instrument is a tabletop instrument for rapid, cross-contamination-free (see Figures 1 and 2) preparation of nucleic acids and PCR setup. Equipped with a reliable robotic system, it can process up to 32 different samples in one batch. The system meets the demanding nucleic acid isolation needs of all of your research applications. Automatically isolate any type of nucleic acid. The magnetic-bead technology, combined with dedicated kits, enables consistent isolation of high-quality DNA or RNA.
The MagNA Pure LC 2.0 Instrument can precisely set up your downstream PCR/RT-PCR reactions. The post-elution part of the MagNA Pure LC 2.0 Instrument is flexible enough to allow utilization of the nucleic acids in a variety of different PCR reaction formats. Isolated nucleic acids and master mixes can be automatically pipetted into LightCycler® Capillaries, 96-well PCR plates, A-rings, or tubes.
- Process samples from a wide variety of sources. Easily isolate nucleic acids from whole blood, white blood cells, bacteria, tissue, and other difficult samples.
- Experience fast isolation and easy setup. Simple, software-guided handling steps launch the automated isolation procedure, processing up to 32 samples in as little as 60 minutes.
- Rely on our high-quality reagents. High-quality kits and accessories provide consistent results that guarantee your success.
- Let the instrument set up your experiment. Following nucleic acid isolation, the unique, programmable post-elution unit automatically pipets sample and reagents, such as for PCR on the LightCycler® Instrument or 96-well block cycler-based PCR reactions.
- Experience outstanding reproducibility and scalability. Obtain consistent yield (see Figures 3 and 4) and recover your purified nucleic acids with high efficiency, regardless of the volume of starting material (see Figure 5).
- Utilize network connectivity features for easy data management. Communicate with your laboratory network or LIMS to download sample input data directly into purification setup and upload subsequent run information.
- Improved user convenience. New instrument hardware features include integrated PC, touchscreen, new interfaces and device, solid waste management, hardware-coded cooling block for a LightCycler® 96-well PCR plate, etc.
- MagNA Pure LC 2.0 Instrument
- Inbuilt PC with Touch Screen
- MagNA Pure LC Cooling Block, LC Centrifuge Adapters
- MagNA Pure LC Cooling Block, 96-well PCR Plate
- LightCycler® 480 PCR Plate Adapter
- Touchscreen Pen
- MagNA Pure LC 2.0 Post-Elution Software
- MagNA Pure LC 2.0 Operator's Manual
- MagNA Pure LC Greasing Set
- O-Ring Exchange Tool
The MagNA Pure LC 2.0 Instrument, kits, reagent design, and software protocols permit processing of virtually all relevant types of sample material. Nucleic acids (e.g., cellular, viral, bacterial, or fungal DNA, RNA, or mRNA) from a broad variety of research samples such as whole blood, blood cells, cultured cells, plasma, serum, sputum, stool, BAL, plant tissues, or food products can be purified in approximately one to three hours. Reproducibility and scalability of purification are increased and the risk of cross-contamination is significantly reduced compared to standard manual methods.
|Mammalian blood samples||Tissue samples||Cultured cells||Materials for consumer-health investigations|
|Whole blood||Fresh/frozen tissue||K-562, HeLa, etc.||Plants|
|Serum||Paraffin-embedded tissue||Bacterial cultures|
|Plasma||Formalin-fixed tissue||Food material|
|White blood cells|
|Peripheral blood mononuclear cells (PBMCs)|
|Body fluids (bronchoalveolar lavage [BAL], sputum, cerebrospinal fluid, urine, swabs)|
Cross-contamination studies on the MagNA Pure LC 2.0 Instrument were performed with 32 blood samples. In an alternating positive and negative (checkerboard) pattern, half of the samples in the cartridge were spiked with Parvovirus B19 DNA (Figure 1). Under these conditions, the risk of cross-contamination of the Parvovirus-negative samples is very high. The isolates were analyzed for the presence of Parvovirus DNA by PCR on the LightCycler® Instrument (Figure 2). This PCR system detects less than 10 Parvovirus DNA copies. The results confirmed that none of the 16 negative controls per run turned out positive. For 10 isolation runs, the same results were always obtained.
Figure 1: MagNA Pure LC nucleic acid isolates were analyzed on the LightCycler® Instrument, followed by agarose gel electrophoresis. In each row of 8 samples, every second well was spiked with Parvovirus B19 DNA. Parvovirus-specific fragments (259 bp) appeared in all lanes of the spiked samples, and in none of the lanes of the non-spiked samples.
Figure 2: The LightCycler® System analysis of the same PCR products as in Figure 1 resulted in two clearly distinguishable bundles of curves, 16 positive and 16 negative samples, corresponding to the spiked and non-spiked cartridge wells.
In one run, 32 isolations of genomic DNA from human whole blood were performed. The isolated DNA was run on an agarose gel (Figure 3) and analyzed by amplification of the Cyclophilin A gene on the LightCycler® Instrument (Figure 4). The results clearly demonstrate that there is no visible intra-assay variance in nucleic acid isolation performed with the MagNA Pure LC 2.0 Instrument.
Figure 3: Thirty-two DNA isolations were run by electrophoresis on a 0.4% agarose gel, showing the same intensity of all bands.
Figure 4: Thirty-two DNA isolations were analyzed by amplification of the Cyclophilin A gene on the LightCycler® Instrument using HybProbe probes. The analysis clearly shows congruent curves for all amplifications.
Three samples of white blood cells (WBCs) were recovered, each from 100 µl, 200 µl, 400 µl, and 600 µl of human whole blood. The mRNA was isolated from these WBCs and analyzed by the amplification of Cyclophilin A on the LightCycler® Instrument. The results shown in Figure 5 demonstrate the precise reproducibility of yields and scalability of isolations done with the MagNA Pure LC 2.0 Instrument.
Figure 5: Reproducibility of yields and scalability of mRNA isolations. Four bundles of curves, each representing three identical isolations can be clearly distinguished. From left to right, the bundles show the results of the analysis of mRNA isolations from WBCs recovered from 600 µl, 400 µl, 200 µl, and 100 µl of human whole blood.
The MagNA Pure LC system benefits from dedicated high-quality reagents and kits. User-friendly software protocols adapt to your specific sample requirements. An option for automated, programmable pipetting of post-isolation PCR setup helps you set up your experiment.
Sample ID and information can be entered by typing or via a barcode reader into a prepared table in the software operating the MagNA Pure LC 2.0 Instrument. After entering the information of the correct MagNA Pure LC Kit and the appropriate purification protocol*, the amounts of reagents and the number of reaction tips required for the run are automatically calculated by the software. All required reagents are filled into nuclease-free, disposable Reagent Tubs; samples are loaded into Sample Cartridges with a capacity of 32 wells and set in place by the user. All subsequent steps are performed automatically with the specifically designed nuclease-free disposable Reaction Tips. These tips are used for pipetting the reagents and as reaction vials for the separation of nucleic acids using magnetic beads, d uring the washing steps, and elution of nucleic acids from the magnetic glass particles into the cooled Sample Cartridge. Used reaction tips are automatically discarded into an attached autoclavable Waste Bag and liquid waste is pipetted at the end of the run into an appropriate Waste Tank.
* Depends on the sample material, the nucleic acid to be purified, the number of isolations, and the sample and elution volumes.
The MagNA Pure LC 2.0 Instrument is designed with a variety of intelligent features for rapid, cross-contamination-free preparation of nucleic acids. A completely closed housing unit, automatic clot and tip loss detection, as well as sample tracking, makes the MagNA Pure LC 2.0 Instrument a true "walk-away" instrument for general laboratory use. Filtration, centrifugation, and any other manual steps are completely eliminated. The MagNA Pure LC 2.0 Instrument utilizes a controlled, piston-driven 8-nozzle pipetting head and positive displacement without vacuum pumps or tubing, and, therefore, the risk of cross-contamination is reduced to an absolute minimum. The inside of the housing unit can easily be cleaned with commonly used disinfectants and decontaminated with a built-in UV-lamp. The UV decontamination and the HEPA-filter system guarantee safety for both samples and environment. The instrument allows the use of a barcode scanner and barcode printer as well as to download sample information from a LIMS or network (file sharing). Run result data can also be uploaded to a LIMS or network. The instrument hardware offers user-convenient features like an integrated PC, touchscreen, new interfaces and device, solid waste management, hardware coded cooling block for a LightCycler® 96 well PCR plate, etc.
|Type||Standalone tabletop instrument|
|Dimensions||108 cm x 77cm x 91 cm
40 in x 26 in x 35 in
(W x D x H)
|Weight||170 kg (375 lbs)|
|Computer||Inbuilt PC with Touch Screen, keyboard and pen|
|Sample Number||1 - 32 tests/batch|
|Dispensable Volume||5 - 1,000 μL|
|Dispensing Accuracy||5 to 100 μL: <3% CV
50 to 1,000 μL: <2% CV
|User Interface||User-friendly interface|
|Data Management||LIMS connectivity, file sharing ability|
|Ports/ Drives||USB, LAN,CD drive|
|Power Source||110 - 120 V AC, at 10 A and 50/60 Hz
220 - 240 V AC, at 5 A and 50/60 Hz
|Power Consumption||Max. 1,000 VA|
The MagNA Pure LC 2.0 Instrument utilizes a proven magnetic-bead technology for the isolation process. This technology has been successfully used throughout the world to generate research success and numerous publications over the last ten years. The choice of nucleic acid purification protocol depends on the sample type and the specific application.
Click here to learn more about the MagNA Pure LC 2.0 System Technology.
Protocols for MagNA Pure LC Instruments
MagNA Pure LC 1.0 (MagNA Pure LC) Instrument (SW v 3.011)
> Download and Installation Instruction (74 KB PDF-File)
Protocols for Total Nucleic Acid Kit - High Performance, up to 200 µL sample volume (24 KB ZIP-File)
Protocols for Total Nucleic Acid Kit - High Performance, 500 µL sample volume (13 KB ZIP-File)
Protocols for Total Nucleic Acid Kit - High Performance, 1,000 µL sample volume (8 KB ZIP-File)
MagNA Pure LC 2.0 Instrument (SW v 1.1.24)
> Download and Installation Instruction (25 KB PDF-File)
Protocols for Total Nucleic Acid Kit - High Performance, up to 200 µL sample volume (26 KB ZIP-File)
Protocols for Total Nucleic Acid Kit - High Performance, 500 µL sample volume (15 KB ZIP-File)
Protocols for Total Nucleic Acid Kit - High Performance, 1,000 µL sample volume (10 KB ZIP-File)
Note: If a software version earlier than v 1.1.24 is installed on your MagNA Pure LC Instrument, please contact Roche prior to installation of these protocols to get an update to v 1.1.24.