RealTime ready Human Reference Gene Panel
Panel of qPCR assays for use on the LightCycler® 480 Instrument to quantify expression of 19 human reference genes.
For life science research only. Not for use in diagnostic procedures.
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To order these products, please contact your Roche order management team.
The panel consists of 19 pretested, ready-to-use qPCR reference gene assays, supplied in a LightCycler® 480 Multiwell Plate 96, white. Each assay includes appropriate primers and a Universal ProbeLibrary probe that contains Locked Nucleic Acid (LNA). The only components that need to be added are sample cDNA and reaction mix (e.g., LightCycler® 480 Probes Master).
Each reference gene assay is repeated four times on a 96-well plate. In addition to the reference gene assays, each set of assays includes three positive- and two negative control assays for the reverse transcription (RT) reaction. The RT positive control is used to determine if degradation of your initial RNA sample has occurred and to assess the quality of your RT step. The RT negative control is used to detect residual genomic DNA.
The 19 human reference genes that can be assayed with the panel include: 18S, ACTB (β-actin), ALAS, β2M, β-globin, G6PD, GAPDH, GUSB (β-glucuronidase), HPRT1, IPO8, PBGD, PGK1, PPIA (cyclophilin A), RPL13A, RPLP0 (large ribosomal protein), SDHA, TBP (TATA-box binding protein), TFRC, and YWHAZ.
- Be confident in your results:
Controls are provided and assays are pretested to ensure optimal performance.
- Save time with a fast and efficient workflow:
The convenient pre-plated assays require only the addition of sample cDNA and master mix.
- Easily import sample information directly to the LightCycler® 480 Instrument:
Plate configuration details can be accessed online and downloaded to the LightCycler® 480 Software.
One package containing two LightCycler® 480 Multiwell Plates 96, white and Sealing Foils. Each plate contains 19 human reference gene assays, three positive- and two negativecontrol assays for the reverse transcription (RT) reaction. The RT positive control is used to determine if degradation of your initial RNA sample has occurred and to assess the quality of your RT step. The RT negative control is used to detect residual genomic DNA.
Each assay is provided in quadruplicate on the 96-well plate.
For the plate layout, please see the attached pdf 'Plate Layout 96' . To import the sample information to your LightCycler® 480 software 1.5 sample editor, please open the attached file 'Macros Reference Genes 96.zip' to either download the LightCycler® 480 Experiment Macro, appropriate for your LightCycler® 480 System I or II, containing the sample information and a complete run protocol or the txt. file containing the sample information only.
The RealTime ready Human Reference Gene Panel allows you to quantify the expression levels of 19 human genes that can be used as reference genes. This panel can be assayed directly on a LightCycler® 480 Instrument.
A reference gene is used to determine expression levels of other genes by relative quantification. If one of the reference genes in the panel is expressed at a level similar to those of the genes you are studying, it can be useful for relative quantification of your genes.
Additional RealTime ready Focus Panels:
Housekeeping or reference genes are typically constitutive genes that are transcribed at a constant level. The expression of these genes are commonly used to normalize for possible variations in the amount and quality of RNA between different samples. Although the expression of the housekeeping genes is constant under most conditions, some cell treatments may lead to variations. An accurate selection of the reference genes, stable under experimental conditions, is essential for reliable results.