{ "ProductData": { "ID": "3.6.8.50.1.15", "ProductType": "Others", "BrandName": "High Pure FFPET DNA Isolation Kit", "ProductNameAddition": "", "RegulatoryDisclaimer1": "For life science research only. Not for use in diagnostic procedures.", "DisclaimerGroup1": "LSR", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ "Formalin-Fixed Paraffin-Embedded Tissue (FFPET)" ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "High Pure FFPET DNA Isolation Kit" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Article_06650767001_im_en.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "06-0008", "StructureNodeStatus": "valid", "NodeName": "High Pure" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "20-000-00", "StructureNodeStatus": "valid", "NodeName": "Assays Reagents and Strips" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "200", "StructureNodeStatus": "valid", "NodeName": "High Pure" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "130-00", "StructureNodeStatus": "valid", "NodeName": "Sample Preparation" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "150-00", "StructureNodeStatus": "valid", "NodeName": "Research Lab" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "100", "StructureNodeStatus": "valid", "NodeName": "Molecular" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "170-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "06650767001", "MaterialDescription": "High Pure FFPET DNA Isolation Kit", "RegisteredProductName": "High Pure FFPET DNA Isolation Kit", "GTIN": "04038377028484", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 kit", "PackSizeDescPIM360": "50 isolations", "MaterialAnnotation": "", "ReadyForUse": "", "OrderInformation": "" } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Storage Conditions (Product)", "Value": "Store the kit at +15 to +25°C.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Storage Conditions (Working Solution)", "Value": "
Solution Storage
Wash Buffer I +15 to +25°C
Wash Buffer II +15 to +25°C
Proteinase K -15 to -25°C
", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Working Solution)" }, { "Name": "Applications", "Value": "The High Pure FFPET DNA Isolation Kit uses a fast and optimized technology for the isolation and purification of genomic DNA from formalin-fixed, paraffin-embedded tissue research samples. The quality of DNA from tissue samples is suitable for the following downstream applications:
  • Quantitative PCR (qPCR)
  • Next generation Sequencing (NGS)
", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Product Characteristics", "Value": "Kit for manual DNA isolation from FFPE tissue for subsequent analysis in PCR, arrays and NG-sequencing.", "Language": "en", "Country": "XG", "Code": "Product Characteristics" }, { "Name": "Assay Time", "Value": "
Total time Approx. 3 hours including deparaffinization procedure
", "Language": "en", "Country": "XG", "Code": "Assay Time" }, { "Name": "Content", "Value": "
Vial / BottleCapLabelFunction / DescriptionContent
1whiteDNA Tissue Lysis Buffer
  • For the lysis of cells
10 ml
2pinkProteinase K, PCR grade
  • Lyophilizate
  • For sample homogenization and inactivation of endogenous nucleases
100 mg
3greenDNA Binding Buffer
  • Contains 5 M guanidine HCl
  • Store protected from light
25 ml
4blackWash Buffer I
  • Contains 5 M guanidine HCl (final concentration after addition of ethanol)
25 ml, add 15 ml absolute ethanol
5blueWash Buffer II 20 ml, add 80 ml absolute ethanol
6colorlessDNA Elution Buffer 5 × 1,000 μl
7colorlessReagent Preparation Buffer 11.5 ml
8 High Pure Filter Tubes
  • For use of up to 800 μl sample volume
50 polypropylene tubes with two layers of glass fiber fleece
9 Collection Tubes Three bags with 50 polypropylene tubes (2 ml)
All solutions are clear and should not be used when precipitates have formed. Warm the solutions at +15 to +25°C or in a 37°C water bath until the precipitates have dissolved.
The buffers can show a slight yellow color. This will have no impact on the function of the buffer
", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Product Description", "Value": "Kit for manual DNA isolation from FFPE tissue for subsequent analysis in PCR, arrays and NG-sequencing.", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Principle", "Value": "FFPE tissue samples are disrupted and homogenized during incubation with Tissue Lysis Buffer and Proteinase K.  Nucleic Acids (NA) bind in the presence of a chaotropic salt specifically to the surface of glass fibers pre-packed in the High Pure Purification Filter Tube.
DNA is purified in a series of rapid \"wash-and-spin\" steps to remove salts, proteins, and cellular components.
Finally, a low-salt elution releases the DNA from the glass fiber. The process does not require DNA precipitation or organic solvent extraction, ideal for rapidly purifying many samples simultaneously.

  1. Samples are disrupted in Tissue Lysis Buffer and homogenized during an incubation with Proteinase K.
  2. In the presence of chaotropic salt, nucleic acids (NA) bind specifically to the surface of glass fibers pre-packed in the High Pure Filter Tube.
  3. Bound nucleic acids are washed, and thereby purified of salts, proteins, and other impurities.
  4. For the final step, a low-salt elution releases the DNA from the glass fibers.
", "Language": "en", "Country": "XG", "Code": "Principle" } ] } } ] }

High Pure FFPET DNA Isolation Kit

Kit for manual DNA isolation from FFPE tissue for subsequent analysis in PCR, arrays and NG-sequencing.

LSR For life science research only. Not for use in diagnostic procedures.
High Pure FFPET DNA Isolation Kit