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"ID": "3.6.8.50.1.15",
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"BrandName": "High Pure FFPET DNA Isolation Kit",
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"RegulatoryDisclaimer1": "For life science research only. Not for use in diagnostic procedures.",
"DisclaimerGroup1": "LSR",
"RegulatoryDisclaimer2": null,
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"SampleType": [
"Formalin-Fixed Paraffin-Embedded Tissue (FFPET)"
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""
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"Name": "Storage Conditions (Product)",
"Value": "Store the kit at +15 to +25°C.",
"Language": "en",
"Country": "XG",
"Code": "Storage Conditions (Product)"
},
{
"Name": "Storage Conditions (Working Solution)",
"Value": "
| Solution | Storage |
| Wash Buffer I | +15 to +25°C |
| Wash Buffer II | +15 to +25°C |
| Proteinase K | -15 to -25°C |
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"Language": "en",
"Country": "XG",
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"Value": "The High Pure FFPET DNA Isolation Kit uses a fast and optimized technology for the isolation and purification of genomic DNA from formalin-fixed, paraffin-embedded tissue research samples. The quality of DNA from tissue samples is suitable for the following downstream applications:
- Quantitative PCR (qPCR)
- Next generation Sequencing (NGS)
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"Name": "Product Characteristics",
"Value": "Kit for manual DNA isolation from FFPE tissue for subsequent analysis in PCR, arrays and NG-sequencing.",
"Language": "en",
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"Code": "Product Characteristics"
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"Name": "Assay Time",
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| Total time | Approx. 3 hours including deparaffinization procedure |
|---|
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| Vial / Bottle | Cap | Label | Function / Description | Content |
|---|
| 1 | white | DNA Tissue Lysis Buffer | | 10 ml |
| 2 | pink | Proteinase K, PCR grade | - Lyophilizate
- For sample homogenization and inactivation of endogenous nucleases
| 100 mg |
| 3 | green | DNA Binding Buffer | - Contains 5 M guanidine HCl
- Store protected from light
| 25 ml |
| 4 | black | Wash Buffer I | - Contains 5 M guanidine HCl (final concentration after addition of ethanol)
| 25 ml, add 15 ml absolute ethanol |
| 5 | blue | Wash Buffer II | | 20 ml, add 80 ml absolute ethanol |
| 6 | colorless | DNA Elution Buffer | | 5 × 1,000 μl |
| 7 | colorless | Reagent Preparation Buffer | | 11.5 ml |
| 8 | | High Pure Filter Tubes | - For use of up to 800 μl sample volume
| 50 polypropylene tubes with two layers of glass fiber fleece |
| 9 | | Collection Tubes | | Three bags with 50 polypropylene tubes (2 ml) |
All solutions are clear and should not be used when precipitates have formed. Warm the solutions at +15 to +25°C or in a 37°C water bath until the precipitates have dissolved.
The buffers can show a slight yellow color. This will have no impact on the function of the buffer
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"Name": "Product Description",
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"Country": "XG",
"Code": "Product Description"
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"Name": "Principle",
"Value": "FFPE tissue samples are disrupted and homogenized during incubation with Tissue Lysis Buffer and Proteinase K. Nucleic Acids (NA) bind in the presence of a chaotropic salt specifically to the surface of glass fibers pre-packed in the High Pure Purification Filter Tube.
DNA is purified in a series of rapid \"wash-and-spin\" steps to remove salts, proteins, and cellular components.
Finally, a low-salt elution releases the DNA from the glass fiber. The process does not require DNA precipitation or organic solvent extraction, ideal for rapidly purifying many samples simultaneously.
- Samples are disrupted in Tissue Lysis Buffer and homogenized during an incubation with Proteinase K.
- In the presence of chaotropic salt, nucleic acids (NA) bind specifically to the surface of glass fibers pre-packed in the High Pure Filter Tube.
- Bound nucleic acids are washed, and thereby purified of salts, proteins, and other impurities.
- For the final step, a low-salt elution releases the DNA from the glass fibers.
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