{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For life science research only. Not for use in diagnostic procedures.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "High Pure FFPET RNA Isolation Kit", "DisclaimerGroup1": "LSR", "ControlTissue": [ "" ], "BrandName": "High Pure FFPET RNA Isolation Kit", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.6.8.50.1.14", "ProductNameAddition": "Kit for manual RNA isolation from FFPE tissue for subsequent analysis in PCR, arrays and NG-sequencing.", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Article_06650775001_im_en.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "High Pure", "NodeID": "06-0008" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Assays Reagents and Strips", "NodeID": "20-000-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "High Pure", "NodeID": "200" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "Sample Preparation", "NodeID": "130-00" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Research Lab", "NodeID": "150-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "Molecular", "NodeID": "100" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "LifeScience", "NodeID": "170-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "High Pure", "NodeID": "06-0008" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Assays Reagents and Strips", "NodeID": "20-000-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "High Pure", "NodeID": "200" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "Sample Preparation", "NodeID": "130-00" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Research Lab", "NodeID": "150-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "Molecular", "NodeID": "100" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "LifeScience", "NodeID": "170-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "06650775001", "MaterialDescription": "High Pure FFPET RNA Isolation Kit", "RegisteredProductName": "High Pure FFPET RNA Isolation Kit", "GTIN": "04038377028491", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 kit", "PackSizeDescPIM360": "50 isolations", "MaterialAnnotation": "", "ReadyForUse": "", "OrderInformation": "" } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Store the kit at +15 to +25°C.", "Country": "XG", "Code": "Storage Conditions (Product)", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "
Solution Storage
Wash Buffer I +15 to +25 °C
Wash Buffer II +15 to +25 °C
Proteinase K -15 to -25°C
DNase I (reconstituted) -15 to -25°C
", "Country": "XG", "Code": "Storage Conditions (Working Solution)", "Name": "Storage Conditions (Working Solution)" }, { "Language": "en", "Value": "Kit for manual RNA isolation from FFPE tissue for subsequent analysis in PCR, arrays and NG-sequencing.", "Country": "XG", "Code": "Product Characteristics", "Name": "Product Characteristics" }, { "Language": "en", "Value": "The High Pure FFPET RNA Isolation Kit uses a fast and optimized technology for the isolation and purification of total RNA from formalin-fixed, paraffin-embedded tissue research samples. The quality of RNA from tissue samples is suitable for the following downstream applications:
  • RT-PCR
  • Gene Expression Array
  • Next Generation Sequencing
  • PreAmplification
", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "
Vial / Bottle Cap Label Function / Description Content
1 red RNA Tissue Lysis Buffer
  • For the lysis of cells
6 ml
2 pink  Proteinase K, PCR grade
  • Lyophilizate
  • For sample homogenization and inactivation of endogenous nucleases
2 × 100 mg
3 green  RNA Binding Buffer
  • Contains 5 M guanidine thiocyanate
Store protected from light
20 ml
4 black Wash Buffer I
  • Contains 5 M guanidine HCl (final concentration after addition of ethanol)
25 ml, add 15 ml absolute ethanol
5 blue Wash Buffer II   20 ml, add 80 ml absolute ethanol
6 white DNase I
  • Lyophilizate
  • Contains 4 kU DNAse I
  • For digestion of residual DNA
100 mg
 
7 white DNase Incubation Buffer (1x)   6 ml
8 colorless RNA Elution Buffer
  • Water, PCR Grade
5 × 1,000 μl
9 colorless Reagent Preparation Buffer   11.5 ml
10   High Pure Filter Tubes   50 polypropylene tubes with two layers of glass fiber fleece
11   Collection Tubes   3 bags containing 50 polypropylene tubes (2 ml)
All solutions are clear and should not be used when precipitates have formed. Warm the solutions at +15 to +25°C or in a 37°C water bath until the precipitates have dissolved.
The buffers can show a slight yellow color. This will have no impact on the function of the buffer
", "Country": "XG", "Code": "Content", "Name": "Content" }, { "Language": "en", "Value": "Kit for manual RNA isolation from FFPE tissue for subsequent analysis in PCR, arrays and NG-sequencing.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "
Total timeApprox. 2.5 hours including deparaffinization procedure
", "Country": "XG", "Code": "Assay Time", "Name": "Assay Time" }, { "Language": "en", "Value": "FFPE tissue samples are disrupted and homogenized during incubation with RNA Tissue Lysis Buffer and Proteinase K. Nucleic Acids (NA) bind in the presence of a chaotropic salt specifically to the surface of glass fibers pre-packed in the High Pure Purification Filter Tube.
RNA is purified in a series of rapid \"wash-and-spin\" steps to remove salts, proteins, and cellular components. Residual DNA is digested on the column by adding DNase I.
Finally, a low-salt elution releases the RNA from the glass fiber. The process does not require RNA precipitation or organic solvent extraction, ideal for rapidly purifying many samples simultaneously.

  1. Samples are disrupted in RNA Tissue Lysis Buffer and homogenized during an incubation with Proteinase K.
  1. In the presence of chaotropic salt, nucleic acids (NA) bind specifically to the surface of glass fibers pre-packed in the High Pure Filter Tube.
  2. To eliminate residual DNA, an on-column DNase I digestion is performed
  3. Bound RNA is washed, and thereby purified of salts, proteins, and other impurities.
  4. For the final step, a low-salt elution releases the RNA from the glass fibers.
", "Country": "XG", "Code": "Principle", "Name": "Principle" } ] } } ] }

High Pure FFPET RNA Isolation Kit

Kit for manual RNA isolation from FFPE tissue for subsequent analysis in PCR, arrays and NG-sequencing.

High Pure FFPET RNA Isolation Kit