{ "ProductData": { "ID": "3.6.8.50.1.14", "ProductType": "Others", "BrandName": "High Pure FFPET RNA Isolation Kit", "ProductNameAddition": "", "RegulatoryDisclaimer1": "For life science research only. Not for use in diagnostic procedures.", "DisclaimerGroup1": "LSR", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ "Formalin-Fixed Paraffin-Embedded Tissue (FFPET)" ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "High Pure FFPET RNA Isolation Kit" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Article_06650775001_im_en.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "06-0008", "StructureNodeStatus": "Active", "NodeName": "High Pure" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "20-000-00", "StructureNodeStatus": "Active", "NodeName": "Assays Reagents and Strips" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "200", "StructureNodeStatus": "Active", "NodeName": "High Pure" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "130-00", "StructureNodeStatus": "Active", "NodeName": "Sample Preparation" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "150-00", "StructureNodeStatus": "Active", "NodeName": "Research Lab" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "100", "StructureNodeStatus": "Active", "NodeName": "Molecular" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "170-00", "StructureNodeStatus": "Inactive", "NodeName": "" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "99-00-00", "StructureNodeStatus": "Inactive", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "Inactive", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "Inactive", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "Inactive", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "230", "StructureNodeStatus": "Active", "NodeName": "LightCycler" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "06650775001", "MaterialDescription": "High Pure FFPET RNA Isolation Kit", "RegisteredProductName": "High Pure FFPET RNA Isolation Kit", "GTIN": "04038377028491", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 kit", "PackSizeDescPIM360": "50 isolations", "MaterialAnnotation": "", "ReadyForUse": "", "OrderInformation": "" } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Storage Conditions (Product)", "Value": "Store the kit at +15 to +25°C.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Storage Conditions (Working Solution)", "Value": "

Solution	Storage
Wash Buffer I	+15 to +25 °C
Wash Buffer II	+15 to +25 °C
Proteinase K	-15 to -25°C
DNase I (reconstituted)	-15 to -25°C

", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Working Solution)" }, { "Name": "Product Characteristics", "Value": "Kit for manual RNA isolation from FFPE tissue for subsequent analysis in PCR, arrays and NG-sequencing.", "Language": "en", "Country": "XG", "Code": "Product Characteristics" }, { "Name": "Applications", "Value": "The High Pure FFPET RNA Isolation Kit uses a fast and optimized technology for the isolation and purification of total RNA from formalin-fixed, paraffin-embedded tissue research samples. The quality of RNA from tissue samples is suitable for the following downstream applications:

• RT-PCR
• Gene Expression Array
• Next Generation Sequencing
• PreAmplification

", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Content", "Value": "

Vial / Bottle	Cap	Label	Function / Description	Content
1	red	RNA Tissue Lysis Buffer	For the lysis of cells	6 ml
2	pink	Proteinase K, PCR grade	Lyophilizate For sample homogenization and inactivation of endogenous nucleases	2 × 100 mg
3	green	RNA Binding Buffer	Contains 5 M guanidine thiocyanate Store protected from light	20 ml
4	black	Wash Buffer I	Contains 5 M guanidine HCl (final concentration after addition of ethanol)	25 ml, add 15 ml absolute ethanol
5	blue	Wash Buffer II		20 ml, add 80 ml absolute ethanol
6	white	DNase I	Lyophilizate Contains 4 kU DNAse I For digestion of residual DNA	100 mg
7	white	DNase Incubation Buffer (1x)		6 ml
8	colorless	RNA Elution Buffer	Water, PCR Grade	5 × 1,000 μl
9	colorless	Reagent Preparation Buffer		11.5 ml
10		High Pure Filter Tubes		50 polypropylene tubes with two layers of glass fiber fleece
11		Collection Tubes		3 bags containing 50 polypropylene tubes (2 ml)

", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Product Description", "Value": "Kit for manual RNA isolation from FFPE tissue for subsequent analysis in PCR, arrays and NG-sequencing.", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Assay Time", "Value": "

Total time	Approx. 2.5 hours including deparaffinization procedure

", "Language": "en", "Country": "XG", "Code": "Assay Time" }, { "Name": "Principle", "Value": "FFPE tissue samples are disrupted and homogenized during incubation with RNA Tissue Lysis Buffer and Proteinase K. Nucleic Acids (NA) bind in the presence of a chaotropic salt specifically to the surface of glass fibers pre-packed in the High Pure Purification Filter Tube.
RNA is purified in a series of rapid \"wash-and-spin\" steps to remove salts, proteins, and cellular components. Residual DNA is digested on the column by adding DNase I.
Finally, a low-salt elution releases the RNA from the glass fiber. The process does not require RNA precipitation or organic solvent extraction, ideal for rapidly purifying many samples simultaneously.

1. Samples are disrupted in RNA Tissue Lysis Buffer and homogenized during an incubation with Proteinase K.

1. In the presence of chaotropic salt, nucleic acids (NA) bind specifically to the surface of glass fibers pre-packed in the High Pure Filter Tube.
2. To eliminate residual DNA, an on-column DNase I digestion is performed
3. Bound RNA is washed, and thereby purified of salts, proteins, and other impurities.
4. For the final step, a low-salt elution releases the RNA from the glass fibers.

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