Vial / Bottle | Cap | Label | Function/Description | Content |
---|---|---|---|---|
1 | yellow | LightCycler® 480 Genotyping Master, 5x conc. |
| 4 vials, 384 μL each |
2 | colorless | LightCycler® 480 Genotyping Master, Water, PCR Grade | To adjust the final reaction volume. | 4 vials, 1 mL each |
3 | blue | LightCycler® 480 Genotyping Master, MgCl2, 25 mM | To adjust MgCl2 concentration, if necessary. | 1 vial, 1 mL |
Ready-to-use hot start reaction mix for PCR, followed by melting curve analysis for genotyping using the LightCycler® 480 System.