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1. Plates in resealable, nonsterile plastic bags
2. Sealing Foils are supplied in a separate box also contained in the plate pack

Note: Because of the special characteristics of the LightCycler^® 480 Instrument’s thermal block, optimal assay performance according to the LightCycler^® 480 System’s specifications can only be achieved when LightCycler^® 480 Multiwell Plates are used. Sealing foils compatible with LightCycler^® 480 Multiwell Plates 96 and LightCycler^® 480 Multiwell Plates 384 are also available separately.
For FRET (HybProbe) probes or high resolution melting, LightCycler^® 480 Multiwell plates, white (96-well and 384-well) are recommended.", "Country": "XG", "Code": "Content", "Name": "Content" }, { "Language": "en", "Value": "LightCycler^® 480 Multiwell Plates 384, clear, are used for SYBR Green I and hydrolysis probes-based assays on the LightCycler^® 480 Instrument, 384-well version. They are not recommended for applications based on FRET (HybProbe) probes or high resolution melting.
Note: Clear LightCycler^® 480 Multiwell plates can only be used with LightCycler^® 480 Instruments on which LightCycler^® 480 Software, Version 1.5 is installed.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "The LightCycler^® 480 System is a high performance, plate-based, thermal block cycler based on heating and cooling principles. The innovative design of the thermal block also requires tailor-made reaction devices which match the exact geometry of the block and permit optimal heat transfer.

Note: Due to the special characteristics of the LightCycler^® 480 Instrument's thermal block, optimal assay performance according to the LightCycler^® 480 System's specifications can only be achieved when LightCycler^® 480 Multiwell Plates are used. Sealing Foils compatible with LightCycler^® 480 Multiwell Plates 96 and 384 are also available separately.
For HybProbe probes (FRET) or high resolution melting, LightCycler^® 480 Multiwell plates, white (96-well and 384-well) are recommended.

Note: Before loading the PCR multiwell plate into the LightCycler^® 480 Instrument, it has to be sealed properly with the self-adhesive Sealing Foil by pressing it firmly to the plate surface using the Sealing Foil Applicator provided with the instrument. When sealing the multiwell plate, apply the Sealing Foil Applicator several times using sufficient pressure, especially for those wells at the outer edge of the plate.
Sealing the plate is crucial to eliminate evaporation at high temperatures. Use only the recommended Sealing Foil.

During Roche production of the sealing foil, DNAses and RNAses are thermally destroyed.
Always centrifuge the filled and sealed plate before loading it into the instrument. The Foil should be applied at the end of the PCR setup, as repeated opening and closing will lead to cross-contamination.


Alternative Seal for the Microplates

Since the geometry of the microplates is compatible with the standard dimensions of multiwell plates, any standard seal, such as plastic PCR caps may be used to cover the plates. However, when the microplates are inserted into the LightCycler^® 480 Instrument, the adhesive LightCycler^® 480 Sealing Foils must be used for the following reasons:
- Transparency of the LightCycler^® 480 Sealing Foils is tested to give the best results when detecting fluorescent signals.
- The Foil is pressed onto the plate with a force of more than 500 N, thus producing a tight seal. The use of a different cover may lead to a leak as it may not fit the plate perfectly.

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