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LifeScience
EvoScript Reverse Transcriptase

EvoScript Reverse Transcriptase

Easy-to-use reaction mix for reverse transcription

For general laboratory use.

Product No. Pack Size
07912315001 50 reactions
Contact Us
Product No. Pack Size
07912323001 200 reactions

To order these products, please contact your Roche order management team.

Keywords: RT-qPCR, Two-step, One-step

For labs performing two step RT-qPCR gene expression applications, the EvoScript Reverse Transcriptase has performance allowing a more sensitive and linear detection of targets in an easy-to-use workflow.
 

Benefits
 

  • Ease of use, convenience, speed, reduced error, reproducibility
  • Result consistency, stability and tolerance of targets of varying secondary structure. Ability to work with difficult templates
  • Ability to detect low copy or rare transcripts for gene expression applications
  • Ability to amplify targets and show good representation of low and high abundant targets in one sample

Contents

 

1 EvoScript Reverse Transcriptase,
Reverse Transcriptase		 Contains Protector RNase Inhibitor and
Enzyme Blend, 10× conc.  		 07 912 315 001
07 912 323 001		 1 vial,  100 μl
2 vials, 100 μl each
2 EvoScript Reverse Transcriptase,
Reaction Buffer		 dNTPs, Mg(OAc)2, and salts, 5× conc. 07 912 315 001
07 912 323 001		 1 vial,  200 μl
2 vials, 200 μl each
3 Water, PCR grade To adjust the final reaction volume.
 07 912 315 001
07 912 323 001		 1 vial,  1 ml
2 vials, 1 ml each

The EvoScript Reverse Transcriptase is designed for highly sensitive and convenient cDNA synthesis in two-step real-time RT-qPCR and is compatible with the LightCycler® 480 System, LightCycler® 96 System, or other real-time PCR instruments. The 2-vial composition is ideally suited for reverse transcription, requiring only the addition of gene-specific primers and target RNA.