LifeScience
EvoScript Reverse Transcriptase

EvoScript Reverse Transcriptase

Easy-to-use reaction mix for reverse transcription

For general laboratory use.

Product No. Pack Size
07912315001 50 reactions
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Product No. Pack Size
07912323001 200 reactions

To order these products, please contact your Roche order management team.

Keywords: RT-qPCR, Two-step, One-step

For labs performing two step RT-qPCR gene expression applications, the EvoScript Reverse Transcriptase has performance allowing a more sensitive and linear detection of targets in an easy-to-use workflow.
 

Benefits
 

  • Ease of use, convenience, speed, reduced error, reproducibility
  • Result consistency, stability and tolerance of targets of varying secondary structure. Ability to work with difficult templates
  • Ability to detect low copy or rare transcripts for gene expression applications
  • Ability to amplify targets and show good representation of low and high abundant targets in one sample

Contents

 

1 EvoScript Reverse Transcriptase,
Reverse Transcriptase		 Contains Protector RNase Inhibitor and
Enzyme Blend, 10× conc.  		 07 912 315 001
07 912 323 001		 1 vial,  100 μl
2 vials, 100 μl each
2 EvoScript Reverse Transcriptase,
Reaction Buffer		 dNTPs, Mg(OAc)2, and salts, 5× conc. 07 912 315 001
07 912 323 001		 1 vial,  200 μl
2 vials, 200 μl each
3 Water, PCR grade To adjust the final reaction volume.
 07 912 315 001
07 912 323 001		 1 vial,  1 ml
2 vials, 1 ml each

The EvoScript Reverse Transcriptase is designed for highly sensitive and convenient cDNA synthesis in two-step real-time RT-qPCR and is compatible with the LightCycler® 480 System, LightCycler® 96 System, or other real-time PCR instruments. The 2-vial composition is ideally suited for reverse transcription, requiring only the addition of gene-specific primers and target RNA.