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EvoScript RNA SYBR® Green I Master (ROX)

The EvoScript RNA SYBR® Green I Master (ROX) is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays on instruments requiring ROX dye.

For general laboratory use.

Product No. Pack Size
07912498001 200 reactions

To order these products, please contact your Roche order management team.

The EvoScript RNA SYBR® Green I Master (ROX) as a 1-vial composition, is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.
 

Benefits
 

  • Ease-of-use, convenience, speed, reduced error
  • Result consistency, stability and tolerance of targets of varying secondary structure
  • Ability to detect low copy or rare transcripts for gene expression applications
  • Superior quantification capability for gene expression applications
  • Easier assay optimization
  • Contamination control capability
  • Generate consistent and robust results, no matter what sample type you are starting from or what target you are detecting
  • Rely on a higher sensitivity when detecting low copy numbers, with the input of larger sample volumes into the reaction
  • Obtain fast and specific results

Contents
 

1. EvoScript RNA SYBR® Green I Master (ROX), 5× conc.
2. Water, PCR Grade

The EvoScript RNA SYBR® Green I Master (ROX) is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of primers and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR. In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificit1). The master mix is optimized for SYBR® Green I applications and does not require optimization with Mg(OAc)2.

1) The reverse transcription activity will be suppressed at temperatures significantly below 60°C.