EvoScript Universal cDNA Master
Easy-to-use reaction mix for cDNA synthesis for two-step RT-qPCR
For general laboratory use.
Product No. | Pack Size |
---|---|
07912374001 | 50 reactions |
Product No. | Pack Size |
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07912439001 | 1 kit for up to 100 reactions |
Product No. | Pack Size |
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07912455001 | 200 reactions |
To order these products, please contact your Roche order management team.
The EvoScript Universal cDNA Master provides a convenient solution for time-saving cDNA Synthesis for use in two-step, real-time RT-qPCR. All reagents needed for cDNA synthesis, including primers, nucleotides, buffers, and enzymes are supplied in only two vials, minimizing pipetting efforts. The enzyme blend included in this kit has a broad temperature range and is suitable for high temperature reverse transcription. The amount of random primer and oligo(dT)18 primer included in the EvoScript Universal cDNA Master Reaction Buffer enables high cDNA yields from all regions of the RNA template.
Benefits
- Ease of use, convenience, speed, reduced error, reproducibility
- Result consistency, stability and tolerance of targets of varying secondary structure. Ability to work with difficult templates
- Ability to detect low copy or rare transcripts for gene expression applications
- Ability to amplify targets and show good representation of low and high abundant targets in one sample
Contents
1 | EvoScript Universal cDNA Master Enzyme Mix |
Contains Enzyme Blend for reverse transcription and Protector RNase Inhibitor 10x conc. |
07 912 374 001 07 912 439 001 07 912 455 001 |
1 vial, 100 μl 2 vials, 100 μl each 4 vials, 100 μl each |
2 | EvoScript Universal cDNA Master Reaction Buffer |
Contains random primers, anchored oligo(dT)18, dNTP, and Mg(OAc)2, 5x conc. |
07 912 374 001 07 912 439 001 07 912 455 001 |
1 vial, 200 μl 2 vials, 200 μl each 4 vials, 200 μl each |
3 |
Water, PCR grade | To adjust the final reaction volume. | 07 912 374 001 07 912 439 001 07 912 455 001 |
1 vial, 1 ml 2 vials, 1 ml each 4 vials, 1 ml each |
The EvoScript Universal cDNA Master is designed for highly sensitive and convenient cDNA synthesis for use in two-step RT-qPCR and is compatible with the LightCycler® 480 System, LightCycler® 96 System, or other real-time PCR instruments. The 2-vial composition is ideally suited for reverse transcription, requiring only the addition of target RNA.