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LifeScience
EvoScript Universal cDNA Master

EvoScript Universal cDNA Master

Easy-to-use reaction mix for cDNA synthesis for two-step RT-qPCR

For general laboratory use.

Product No. Pack Size
07912374001 50 reactions
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Product No. Pack Size
07912439001 1 kit for up to 100 reactions
Product No. Pack Size
07912455001 200 reactions

To order these products, please contact your Roche order management team.

Keywords: RT-qPCR, One-step, Two-step

The EvoScript Universal cDNA Master provides a convenient solution for time-saving cDNA Synthesis for use in two-step, real-time RT-qPCR. All reagents needed for cDNA synthesis, including primers, nucleotides, buffers, and enzymes are supplied in only two vials, minimizing pipetting efforts. The enzyme blend included in this kit has a broad temperature range and is suitable for high temperature reverse transcription. The amount of random primer and oligo(dT)18 primer included in the EvoScript Universal cDNA Master Reaction Buffer enables high cDNA yields from all regions of the RNA template.

 

Benefits

 

  • Ease of use, convenience, speed, reduced error, reproducibility
  • Result consistency, stability and tolerance of targets of varying secondary structure. Ability to work with difficult templates
  • Ability to detect low copy or rare transcripts for gene expression applications
  • Ability to amplify targets and show good representation of low and high abundant targets in one sample

 

Contents

 

1 EvoScript Universal cDNA
Master Enzyme Mix		 Contains Enzyme Blend for reverse
transcription and Protector
RNase Inhibitor 10x conc.		 07 912 374 001
07 912 439 001
07 912 455 001		 1 vial,  100 μl
2 vials, 100 μl each
4 vials, 100 μl each
2 EvoScript Universal cDNA Master
Reaction Buffer		 Contains random primers, anchored
oligo(dT)18, dNTP, and Mg(OAc)2,
5x conc.		 07 912 374 001
07 912 439 001
07 912 455 001		 1 vial,  200 μl
2 vials, 200 μl each
4 vials, 200 μl each
3
 		 Water, PCR grade To adjust the final reaction volume. 07 912 374 001
07 912 439 001
07 912 455 001		 1 vial,  1 ml
2 vials, 1 ml each
4 vials, 1 ml each

The EvoScript Universal cDNA Master is designed for highly sensitive and convenient cDNA synthesis for use in two-step RT-qPCR and is compatible with the LightCycler® 480 System, LightCycler® 96 System, or other real-time PCR instruments. The 2-vial composition is ideally suited for reverse transcription, requiring only the addition of target RNA.