High Pure FFPET DNA Isolation Kit
For life science research only. Not for use in diagnostic procedures.
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To order these products, please contact your Roche order management team.
The High Pure FFPET DNA Isolation Kit is designed for the isolation and purification of genomic DNA from 5 to 10 μm thick sections from formalin-fixed, paraffin-embedded tissue. The isolated DNA is suitable for qPCR, microarray analysis (e.g., CGH or methylation arrays), and sequencing.
- Total time required is approximately 3 hours including deparaffinization procedure.
- DNA recovery in 25 to 50 μL elution volume
- Obtain high yields of contamination-free DNA
- DNA Tissue Lysis Buffer
- Proteinase K, PCR-grade
- DNA Binding Buffer
- Wash Buffer I
- Wash Buffer II
- DNA Elution Buffer
- Reagent Preparation Buffer
- High Pure Filter Tubes
The deparaffinized tissue is lysed with a special lysis buffer and incubated with Proteinase K. In the presence of chaotropic salts, the DNA is specifically bound to the glass fiber of the High Pure Filter Tube and purified in a series of rapid wash-and- spin steps and then eluated from the column.