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LightCycler® Color Compensation Set

Ready-to-use solutions for the generation of color-compensation files on the LightCycler® Carousel-Based System Instruments.

For general laboratory use.

Product No. Pack Size
12158850001 5 calibration runs

To order these products, please contact your Roche order management team.

Ready-to-use solutions for the generation of color compensation files for the LightCycler® Carousel-Based System.


  • Blank, standard buffer excluding fluorophores; to determine the standard buffer fluorescence
  • Fluorescein Calibrator, standard oligonucleotide, fluorescein-labeled at the 3' end [0.3 µM]; to determine the crosstalk from fluorescein (channel 1 fluorophore) into channels 2 and 3
  • LightCycler® Red 640 Calibrator, standard oligonucleotide, LightCycler® Red 640-labeled at the 3' end [1 µM]; to determine the crosstalk from LightCycler® Red 640 (channel 2 fluorophore) into channels 1 and 3
  • Cy5.5 Calibrator, standard oligonucleotide, Cy5.5-labeled at the 3' end [1 µM]; to determine the crosstalk from Cy5.5 (channel 3 fluorophore) into channels 1 and 2

The color-compensation files, generated with the LightCycler® Color Compensation Set, are a prerequisite to performing dual-color experiments with both LightCycler® Red 640-labeled and Cy5.5-labeled HybProbe probes in a single capillary.

Although the optical filters of each detection channel of the LightCycler® Instruments are optimized for the different emission maxima of Fluorescein, LightCycler® Red 640, and Cy5.5 dye, some residual crosstalk between the channels is observed. This crosstalk can be corrected using a color-compensation file.


The LightCycler® Color Compensation Set provides ready-to-use solutions for the generation of color-compensation files to compensate for crosstalk. The crosstalk is observed between single channels when using differently labeled HybProbe probes in multiplex dual-color experiments (i.e., in one capillary). The generated color-compensation file can be used in all subsequent dual-color experiments on that specific LightCycler® Carousel-Based System Instrument to compensate for the raw fluorescence data, either online during the run or for data analysis after the run.