LightCycler® Control Kit DNA

Control reaction for PCR using the LightCycler® Carousel-Based System. 

For general laboratory use.

Product No. Pack Size
12158833001 50 x 20 µL reactions

To order these products, please contact your Roche order management team.

The LightCycler® Control Kit DNA includes human genomic DNA as the control template, control primers, HybProbe probes and PCR-grade water for performing PCR using the LightCycler®  Carousel-Based System. It is designed to confirm PCR performance using LightCycler® Carousel-Based Instruments.


  • Human Genomic DNA, (15 ng/µL)
  • β-Globin Primer Mix, 10x concentrated
  • β-Globin HybProbe Mix, LightCycler® Red 640-labeled, 10x concentrated
  • β-Globin HybProbe Mix, Cy5.5-labeled, 10x concentrated
  • Water, PCR Grade 

The LightCycler® Control Kit DNA is designed as a control to confirm PCR-system performance using the LightCycler® Carousel-Based System Instruments, or as a quality check of the human DNA used in a specific assay (when combined with the function-tested primers and HybProbe probes provided with the kit). Alternatively, the kit can be used as a quality check of specific primers and HybProbe probes by using the human genomic DNA provided with the kit. Furthermore, the kit can be used as a functional check of dual-color experiments and color compensation when simultaneously using the provided LightCycler® Red 640- and the Cy5.5-labeled HybProbe probes.

The LightCycler® Control Kit DNA allows control reactions for PCR to be performed using the LightCycler® Carousel-Based System Instruments and the LightCycler® DNA Master Kits, or the LightCycler® FastStart DNA Master Kits.
For detection using SYBR Green I, a 110-bp fragment of the human β-globin gene is amplified with specific primers from human genomic DNA in the presence of this DNA double-strand-specific dye. During online monitoring, the fluorescence increases as a result of the increasing amount of PCR product.
For detection using HybProbe probes, a 110-bp fragment of the human β-globin gene is amplified with specific primers from human genomic DNA.


Function test: The LightCycler® Control Kit DNA is function tested using the LightCycler® DNA Master SYBR Green I and the LightCycler® DNA Master HybProbe, according to the kit protocols. Ten genome equivalents (approximately 30 pg genomic DNA) can be reproducibly detected using the LightCycler® Carousel-Based System.