LightCycler® FastStart DNA Master SYBR Green I

Easy-to-use hot start reaction mix for PCR using the LightCycler® Carousel-Based System.

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size
03003230001 96 x 20 µL reactions
Product No. Pack Size
12239264001 480 x 20 µL reactions

To order these products, please contact your Roche order management team.

Easy-to-use Hot Start Reaction Mix for PCR, using SYBR Green I with the LightCycler® Carousel-Based System.

LightCycler® FastStart DNA Master SYBR Green I provides convenience, high performance, and reproducibility in quantitative PCR.


  • LightCycler® FastStart Enzyme
  • LightCycler® FastStart Reaction Mix SYBR Green
  • MgCl2 Stock Solution, 25 mM
  • Water, PCR Grade 

LightCycler® FastStart DNA Master SYBR Green I is an easy-to-use hot start reaction mix for sensitive PCR applications in LightCycler® capillaries, using SYBR Green I as detection format. They are the ideal master mixes for performing quantitative PCR, as well as SNP and mutation detection. They can also be used in two-step RT-PCR.
Note: LightCycler® FastStart DNA Master SYBR Green I can be used in conjunction with Uracil DNA Glycosylase, heat-labile for carryover prevention during PCR.

SYBR Green I dye is included in the LightCycler® Master Mixes for SYBR Green I detection. During each PCR cycle, SYBR Green I intercalates specifically into the double strand of the amplified PCR products; ongoing amplification is monitored by measuring the increase in fluorescence after each cycle. Combining amplification with melting curve analysis can enhance the specificity and sensitivity of amplification reactions. 
The hot start feature of the master mix is provided by FastStart Taq DNA Polymerase, a chemically modified Taq DNA polymerase, which is inactive at room temperature and becomes activated at high temperatures (i.e., a preincubation step at +95°C for 5 – 10 minutes). If necessary, the MgClconcentration can be optimized for specific applications.
Note: All LightCycler® Master Mixes have been specifically developed to function in LightCycler® capillaries and provide optimal performance and sensitivity.


Function test: The LightCycler® FastStart DNA Master SYBR Green I is function tested using the LightCycler® Control Kit DNA, according to the kit protocols.