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LightCycler® RNA Amplification Kit SYBR Green I

Kit for one-step RT-PCR using the LightCycler® Carousel-Based System.

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size
12015137001 96 x 20 µL reactions

To order these products, please contact your Roche order management team.

The kit provides reagents, including enzyme mix, reaction mix with SYBR Green I dye, MgCl2, and PCR-grade water for amplification and detection of RNA using the LightCycler® Carousel-Based System Instruments.

The LightCycler® RNA Amplification Kit SYBR Green I provides convenience, high performance, reproducibility, and minimized contamination risk. Addition of MgCl2 (provided in the kit) may be necessary to optimize performance.
 

Contents

  • LightCycler® RT-PCR Enzyme Mix
  • LightCycler® RT-PCR Reaction Mix SYBR Green I
  • MgCl2 Stock Solution, 25 mM
  • Water, PCR-grade
  • Resolution Solution

The LightCycler® RNA Amplification Kit SYBR Green I is specifically adapted for one-step RT-PCR in LightCycler® Capillaries using SYBR Green I dye as detection format.

SYBR Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis, the dye binds to the amplified PCR products; the amplicon can be detected by its fluorescence. Combining amplification with melting-curve analysis can enhance specificity and sensitivity of amplification reactions.
 

Quality

The LightCycler® RNA Amplification Kit SYBR Green I is function tested using the LightCycler® Control Kit RNA, according to the kit protocols.