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LightCycler® 480 SYBR Green I Master

Ready-to-use hot start reaction mix for SYBR Green I-based real-time PCR using the LightCycler® 480 Instrument.

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size
04707516001 500 x 20 µL reactions
Product No. Pack Size
04887352001 5000 x 20 µL reactions

To order these products, please contact your Roche order management team.

The LightCycler® 480 SYBR Green I Master is a one-component hot start reaction mix for PCR. It contains FastStart Taq DNA Polymerase and DNA double-strand-specific SYBR Green I dye for product detection and characterization. Since the mix is provided as an easy-to-use real master reagent, reaction setup only requires the addition of template DNA and primers. The mix can be used with different types of DNA (e.g., genomic, cDNA) and is ideally suited for high-throughput applications in 96- or 384-well plates.

 

  • Save time with a convenient, ready-to-use 2x concentrated hot start master mix.
  • Minimize pipetting steps and contamination risk.
  • Perform sensitive, specific, and quantitative PCR or sequence detection using the LightCycler® 480 Instrument.
  • Eliminate time-consuming MgCl2 titration.
  • Achieve consistent, high quality performance with LightCycler® 480 Instruments and Multiwell Plates.

 

Contents

  • LightCycler® 480  SYBR Green I Master, 2x concentrated
  • Water, PCR Grade

 

The LightCycler® 480 SYBR Green I Master provides reagents including SYBR Green I dye, for amplification and detection of DNA using the LightCycler® 480 Instrument. The kit is ideally suited for hot start PCR assays for gene detection and quantification.

SYBR Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis, the SYBR Green I dye, which is included in the reaction mix, binds to the amplified PCR products; the amplicon can be detected by its fluorescence.
Hot start protocols used with the LightCycler® 480 SYBR Green I Master have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can nonspecifically bind. The FastStart Taq DNA Polymerase is “activated” by removing the blocking groups at a high temperature (i.e., a pre-incubation step at +95°C for 5 minutes).
Note: The LightCycler® 480 SYBR Green I Master can be used in conjunction with Uracil-DNA Glycosylase, heat-labile for carryover prevention during PCR. Furthermore, it can be used in two-step RT-PCR applications, for example, when used downstream of Transcriptor Reverse Transcriptase.


Quality

Function test: The LightCycler® 480 SYBR Green I Master is function tested using the LightCycler® 480 Instrument, according to the kit protocols.