LightMix® Modular Parainfluenza 4 (PIV-4)
Kit with reagents for 96 PCR reactions 20 μl for detection of PIV-4 [lyophilized]
TIB MOLBIOL order number: 50-0114-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Human parainfluenza viruses (four serotypes hPIV-1-4) are negative strand ssRNA viruses belonging to the paramyxovirus family. They cause flu-like illness; after the Respiratory Syncytial Virus (RSV) they are the second main cause of hospitalisation of young children suffering from respiratory illness. Infection of immunosuppressed people can cause severe pneumonia which can be fatal.
PIV-4a/b is often not included in testing but are believed to be more common than previously thought, because most children by the age of 10 are sero-positive. PIV-4 causes less often severe disease.
1 Vial orange cap 96 reactions PIV-4 (lyophilized)
1 Vial black cap Positive Control (Cp ~ 30, lyophilized)
Additional Reagents required
LightCycler® Multiplex RNA Virus Master
A 148 bp long fragment from the nucleoprotein (NP) gene is amplified with specific primers and detected with a Cyan500 labeled hydrolysis probe.
This assay detects 10 genome equivalent copies or less per reaction (in vitro transcribed RNA).
Sample Material and Extraction
Typical clinical samples are nasopharyngeal swabs. See ModularDx Document Extraction Protocols.