Kit with reagents for 96 PCR reactions 20 μl for detection of T-cell receptor excision circles [lyophilized]
TIB MOLBIOL order number: 53-0621-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Severe combined immunodeficiency (SCID) belongs to the primary immunodeficiencies (PID) and includes a spectrum of primary immuno-deficiencies of about 14 independent genetic conditions, all of which yield severe defects in cellular and humoral immunity.
Real-time quantitative PCR (qPCR) targeting a specific marker of functional T cells, the T-cell-receptor excision circle (TREC), detects the absence of functional T cells and has a demonstrated clinical validity for detecting severe combined immunodeficiency (SCID) in infants 1.2
1 Vial yellow cap 96 reactions TREC (lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)
Additional Reagents required
LightCycler® Multiplex DNA Master
or LightCycler® 480 Probes Master
1 High-Throughput Multiplex T-Cell-Receptor Excision Circle Quantitative PCR Assay with Internal Controls for Detection of Severe Combined Immunodefficiency in Population–Based Newborn Screening. Gerstel et al., Clin Chem (2010)
2 Neonatal screening for severe primary immunodeficiency diseases using high-throughput triplex real-time PCR. Borte et al,, 2011
An about 100 bp long fragment from T-cell-receptor excision circles is amplified with specific primers and detected with a FAM labeled hydrolysis probe (530 channel).
This assay must be combined with an extraction control, for example MSTN 66-0905-96, Cat. No. 07 225 253 001 (660 label) or 58-0905-96, Cat. No. 07 559 968 001 (580 label). This assay can be combined with KREC 61-0622-96, Cat. No. 07 093 896 001 (610 label).
This assay detects 10 genome equivalent copies or less per reaction (plasmid DNA dilution).
Sample Material and Extraction
Typical specimen are blood from newborn. See ModularDx Document Extraction Protocols