LightMix® Modular Listeria monocytogenes
Kit with reagents for 96 PCR reactions 20 μl for detection of Giardia [lyophilized]
TIB MOLBIOL order number: 53-0642-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Meningitis is an inflammation of the membranes in the brain caused by infection with viruses, bacteria or other microorganisms. For newborn some cases are caused by Listeria monocytogenes, a Gram-positive bacterium better known for foodborne infections.
1 Vial yellow cap 96 reactions Listeria (lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)
Additional Reagents required
LightCycler® Multiplex DNA Master
or LightCycler® 480 Probes Master
A 89 bp fragment of the hlyA gene of L. monocytogenes is amplified with specific primers.and detected with a FAM labeled hydrolysis probe.
This assay detects 10 genome equivalent copies or less per reaction (plasmid DNA dilution).
Sample Material and Extraction
Typical samples are cerebrospinal fluid (CSF). See ModularDx Document Extraction Protocols.