LightMix® Modular Astrovirus
Kit with reagents for 96 PCR reactions 20 μl for detection of Astrovirus [lyophilized]
TIB MOLBIOL order number: 64-0657-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Astrovirus is a common cause for diarrhea and vomiting pain in children, and to less extend in adults. Clinical symptoms are less severe than for Rotavirus and infections can even stay largely asymptomatic. The virus uses the faecal-oral route and may be transferred by contaminated food. The occurrence of infections is highest during the winter months whereas in tropical regions it is linked to the rainy season, probably due to a breakdown of sanitation systems. The virus has a single stranded RNA genome.
There is no vaccine or anti-viral treatment against the Astrovirus infection.
1 Vial blue cap 96 reactions Astrovirus (lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)
Additional Reagents required
LightCycler® Multiplex RNA Virus Master
A 89 bp fragment from the viral capsid gene is amplified with specific primers and and detected with a LC640 labeled hydrolysis probe (640 channel).
This assay detects 10 genome equivalent copies or less per reaction (in vitro transcribed RNA).
Sample Material and Extraction
Typical specimen are from feces samples. See ModularDx Document Extraction Protocols