LightMix® Modular Bocavirus (HBoV)
Kit with reagents for 96 PCR reactions 20 μl for detection of HBoV [lyophilized]
TIB MOLBIOL order number: 58-0120-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Human bocavirus (HBoV) is a ssDNA virus of the family Parvoviridae, which may cause in particular in children lower respiratory tract infections and has been reported to cause gastroenteritis. HBoV is often found together with other viruses known to cause respiratory disease and is common in respiratory samples from persons without symptoms. Four human genotypes HBoV are described.
1 Vial red cap 96 reactions HBoV (lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)
Additional Reagents required
LightCycler® Multiplex DNA Master
or LightCycler® 480 Probes Master or combined with RNA targets : LightCycler® Multiplex RNA Virus Master
A 65 bp long fragment from the viral NP1 gene is amplified with specific primers and detected with a hydrolysis probe with a R6G labelled hydrolysis probe (580 channel).
This assay detects 10 genome equivalent copies or less per reaction (DNA dilution). Since HBoV is part of the Respiratory and Gastro Virus Panels, the test will usually include RT-step.
Sample Material and Extraction
Typical samples are nasopharyngeal aspirates or bronchoalveolar lavage (BAL), for gastrointestinal infections from faeces. See ModularDx Document Extraction Protocols.