LightMix® Modular Bordetella pertussis
Kit with reagents for 96 PCR reactions 20 μl for detection of B. pertussis [lyophilized]
TIB MOLBIOL order number: 58-0679-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Pertussis (whooping cough) is a severe respiratory disease in children caused by a bacterial infection with Bordetella pertussis - the mortality rate is 0.5% in infants under six months. A milder form of the disease is caused by B. parapertussis while B. holmesii and B. bronchiseptica are rare in humans. Common targets for detection of B. pertussis and B. parapertussis are the insertion sequences (IS).
1 Vial red cap 96 reactions B.pertussis (lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)
Additional Reagents required
LightCycler® Multiplex DNA Master
or LightCycler® 480 Probes Master
A 120 bp long fragment from the IS481 gene from B.pertussis is amplified with specific primers and detected with a R6G labeled hydrolysis probe.
This assay detects 10 genome equivalent copies or less per reaction (plasmid DNA dilution).
Sample Material and Extraction
Typical samples are from bronchoalveolare lavage or sputum. See ModularDx Document Extraction Protocols.