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LightMix® Modular Chlamydia pneumoniae

Kit with reagents for 96 PCR reactions 20 μl for detection of Chlamydia pn. [lyophilized]
TIB MOLBIOL order number: 61-0681-96

For life science research only. Not for use in diagnostic procedures. For in vitro use only.

Product No. Pack Size
07730551001 96 reactions

To order these products, please contact your Roche order management team.

Chlamydophila pneumoniae is after Mycoplasma pn. the second most frequent bacterial respiratory pathogen. C. pneumoniae causes endemic and epidemic disease, including pneumonia, bronchitis, pharyngitis and sinusitis. It is commonly spread by respiratory secretions, and has a high incidence rate with an estimated infestation rate of 50 -70%.  
 

Contents

1 Vial yellow cap 96 reactions Mycoplasma pn.(lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)

Additional Reagents required

  • LightCycler® Multiplex DNA Master (if only DNA targets tested)
    or
  • LightCycler® 480 Probes Master

A 85 bp long fragment from the MOMP gene is amplified with specific primers and detected with a LC610 labeled hydrolysis probe.

Specification
This assay detects 10 genome equivalent copies or less per reaction (plasmid DNA dilution).

Sample Material and Extraction
Typical samples are from bronchoalveolare lavage or sputum. See ModularDx Document Extraction Protocols.