LightMix® Modular Dengue Virus
Kit with reagents for 96 PCR reactions 20 μl for detection of Dengue RNA [lyophilized]
TIB MOLBIOL order number 58-0700-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Dengue virus is a plus strand ssRNA virus of the Flavivirus family splitting into 4 serotypes (DENV1-4). Aedes mosquitoes are responsible for transmissions to humans and the disease affects therefore preferentially urban regions with tropical or subtropical climate. About 80% of all Dengue infections are asymptomatic; the others have usually only a few days or weeks of fever, headaches and skin rush.
1 Vial red cap 96 reactions Dengue (lyophilized)
1 Vial black cap Positive Control, Cp-value ~ 30
Additional Reagents required
LightCycler® Multiplex RNA Virus Master
A 116 - 136 bp long fragment (depending on the virus serotype) of the 3-UTR region is amplified with specific primers and detected with a JOE labeled hydrolysis probe (580 channel).
This assay detects 10 genome equivalent copies or less per reaction (in vitro transcripted RNA).
Sample Material and Extraction
Typical specimen are blood or plasma. See ModularDx Document Extraction Protocols.