LightMix® Modular Eae EHEC
Kit with reagents for 96 PCR reactions 20 μl for detection of eae [lyophilized]
TIB MOLBIOL order number: 61-0609-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Escherichia coli is part of the normal flora but some diarrheagenic strains causing severe disease.
Transmission to humans is primarily foodborne. Pathogenic strains are characterized by their toxins.
The enterohaemorrhagic E. coli (EHEC) produce the Attaching and Effacing protein (Eae) and the Shiga-like toxins stx1 and/or stx2 which are closely related to toxins from Shigella dysenteriae.
1 Vial purple cap 96 reactions eae (lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)
Additional Reagents required
LightCycler® Multiplex DNA Master
or LightCycler® 480 Probes Master
A 78 bp long fragment from the eae gene from enterohemorrhagic / enteropathogenic E.coli is amplified with specific primers and detected with a hydrolysis probe with a LC610 labeled hydrolysis probe.
This assay detects 10 genome equivalent copies or less per reaction (plasmid DNA dilution).
Sample Material and Extraction
Typical samples are from feces or bacterial culture. See ModularDx Document Extraction Protocols.