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LightMix® Modular Ebola Virus Zaire

Kit with reagents for 96 PCR reactions 20 μl for detection of Ebola Zaire (2014) RNA [lyophilized]
TIB MOLBIOL order number: 53-0649-96

For life science research only. Not for use in diagnostic procedures. For in vitro use only.

Product No. Pack Size
07383428001 96 reactions

To order these products, please contact your Roche order management team.

Ebola virus (EBOV), known since the first outbreak in Zaire (today Democratic Republic of Congo) in 1976, is a ssRNA Filovirus causing severe disease typically associated with hemorrhagic fever (EHF). Depending on the respective strain the mortality is reported to be between 20% up to more than 90%.
The virus and speicmen must be handled under Level-4 Biosafety conditions. EBOV is listed as potential bioweapon, explaining why detection assays/kits are under EU report restrictions (dual-use-goods).


1 Vial yellow cap 96 reactions Ebola (lyophilized)
1 Vial black cap Positive Control (RNA), Cp-value ~ 31

Additional Reagents required
LightCycler® Multiplex RNA Virus Master

A 127 bp long fragment from the viral RNA-Polymerase (L protein) gene is amplified with specific primers and detected with a FAM labeled hydrolysis probe (530 channel).

Notes :
1 -This kit can be applied with the Multiplex RNA master on LightCycler® 2.0 instruments.
2 -This kit can be combined with the Roche Diagnostics Realtime Ready RNA Virus master.
3 -The annealing temperature can be increased up to 64°C (instructions for 1-3 not included).

This assay detects 10 genome equivalent copies or less per reaction (in vitro transcribed RNA).

Sample Material and Extraction
Typical clinical samples are blood or serum; the virus can be detected also from fecal samples. See ModularDx Document Extraction Protocols.