LightMix® Modular Ebola Virus Zaire
Kit with reagents for 96 PCR reactions 20 μl for detection of Ebola Zaire (2014) RNA [lyophilized]
TIB MOLBIOL order number: 53-0649-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Ebola virus (EBOV), known since the first outbreak in Zaire (today Democratic Republic of Congo) in 1976, is a ssRNA Filovirus causing severe disease typically associated with hemorrhagic fever (EHF). Depending on the respective strain the mortality is reported to be between 20% up to more than 90%.
The virus and speicmen must be handled under Level-4 Biosafety conditions. EBOV is listed as potential bioweapon, explaining why detection assays/kits are under EU report restrictions (dual-use-goods).
1 Vial yellow cap 96 reactions Ebola (lyophilized)
1 Vial black cap Positive Control (RNA), Cp-value ~ 31
Additional Reagents required
LightCycler® Multiplex RNA Virus Master
A 127 bp long fragment from the viral RNA-Polymerase (L protein) gene is amplified with specific primers and detected with a FAM labeled hydrolysis probe (530 channel).
1 -This kit can be applied with the Multiplex RNA master on LightCycler® 2.0 instruments.
2 -This kit can be combined with the Roche Diagnostics Realtime Ready RNA Virus master.
3 -The annealing temperature can be increased up to 64°C (instructions for 1-3 not included).
This assay detects 10 genome equivalent copies or less per reaction (in vitro transcribed RNA).
Sample Material and Extraction
Typical clinical samples are blood or serum; the virus can be detected also from fecal samples. See ModularDx Document Extraction Protocols.