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LightMix® Modular Entamoeba histolytica

Kit with reagents for 96 PCR reactions 20 μl for detection of E. histolytica [lyophilized]
TIB MOLBIOL order number: 50-0611-96

For in vitro diagnostic use.

Product No. Pack Size
07979746001 96 reactions

To order these products, please contact your Roche order management team.

This product is intended to be used to detect (diagnose) parasital genomic DNA from nucleic acid extracts obtained from watery stool sample from patients with signs of diarrhea. This assay has been developed for use in multiplex PCR and can be run with a control reaction alone or combined with other kits as specified in the pack insert.
 

Contents

1 Vial orange cap 96 reactions E.histolytica (lyophilized)
   99.8% oligonucleotides (<0.01 pg)
   0.1% CAS 77-86-1 Tris (hydroxymethyl) aminomethane
   0.1% CAS 60-00-4 Ethylenediamine tetraacetic acid (EDTA)
1 Vial black cap Positive Control, Cp-value ~ 30

Additional Reagents Required
LightCycler® Multiplex DNA Master

For use with Roche '480' instruments. Refer to 'Instructions For Use Gastro Parasites’

Pathogen and Target Information
Entameoba is a parasite which can cause diarrhea. A 90 bp long fragment from the rRNA gene is amplified and detected with a Cyan500 labeled probe.