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LightMix® Modular GES Carbapenemase

Kit with reagents for 96 PCR reactions 20 μl for detection of GES [lyophilized]
TIB MOLBIOL order number: 64-0631-96

For life science research only. Not for use in diagnostic procedures. For in vitro use only.

Product No. Pack Size
07409117001 96 reactions

To order these products, please contact your Roche order management team.

Antibiotics containing a ß-lactam ring like penicillins and cephamycins are commonly used for treatment of Gram-positive as well as some Gram-negative bacteria. Beta-lactamases cleave the β-lactam ring. Carbapenemases, member of the Extended-spectrum ß-lactamases (ESBL) act also on carbapenems. Carbapenem-Resistant Enterobacteriaceae (CRE) are a growing threat for hospital acquired infections.

Guiana extended-spectrum β-lactamase (GES) are named after their first occurrence in French Guiana in Klebsiella pneumoniae. The plasmid-encoded blaGES belong to the ceftazidime-hydrolyzing class A (minor) ESBL; only some GES members exhibit carbapenemase activity. GES 1-9 is seen in particular in Pseudomonas aeruginosa and was found in France, Greece, Southern Africa and South America.
 

Contents

1 Vial blue cap 96 reactions GES (lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)

Additional Reagents required

  • LightCycler® Multiplex DNA Master
    or
  • LightCycler® 480 Probes Master

A 107 bp long fragment from the blaGES gene is amplified with specific primers and detected with a LC640 labeled hydrolysis probe. The assay detects GES 1-9 and 11 while GES-10 will be missed.

Specification
This assay detects 10 genome equivalent copies or less per reaction (plasmid DNA dilution).

Sample Material and Extraction
Typical samples are from feces, rectal swabs or bacterial culture. See ModularDx Extraction Protocols.