LightMix® Modular Haemophilus Influenzae
Kit with reagents for 96 PCR reactions 20 μl for detection of H. influenzae [lyophilized]
TIB MOLBIOL order number: 64-0646-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Haemophilus influenzae are gram-negative bacteria belonging to the family Pasteurellaceae. After virus infections thay may be found as secondary pathogens in the human mucosa. H. influenzae can cause in young children sepsis and bacterial meningitis.
1 Vial blue cap 96 reactions H. influenzae (lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)
Additional Reagents required
LightCycler® Multiplex DNA Master
or LightCycler® 480 Probes Master
A 113 bp long fragment from the hpd gene of Haemophilus influenzae is amplified with specific primers and detected with a LC640 labelled hydrolysis probe.
This assay detects 10 genome equivalent copies or less per reaction (plasmid DNA dilution).
Sample Material and Extraction
For meningitis testing typical samples are cerebrospinal fluid (CSF), for pneumonia testing from bronchoalveolare lavage or sputum. See ModularDx Document Extraction Protocols.