LightMix® Modular IMP Carbapenemase
Kit with reagents for 96 PCR reactions 20 μl for detection of IMP [lyophilized]
TIB MOLBIOL order number: 64-0636-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Antibiotics containing a ß-lactam ring like penicillins and cephamycins are commonly used for treatment of Gram-positive as well as some Gram-negative bacteria. Beta-lactamases cleave the β-lactam ring. Carbapenemases, member of the Extended-spectrum ß-lactamases (ESBL) act also on carbapenems. Carbapenem-Resistant Enterobacteriaceae (CRE) are a growing threat for hospital acquired infections.
The IMP-type carbapenemases are class B metallo-β-lactamases giving rise to Imipenem-resistance first. Currently there are 17 IMP variants described. The blaIMP gene is plasmid-encoded or located on integrons and has be found in Pseudomonas, Acinetobacter as well as Enterobacteriaceae. IMP has been detected worldwide but had the most recent spread to the United States and Australia.
1 Vial blue cap 96 reactions IPM (lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)
Additional Reagents required
LightCycler® Multiplex DNA Master
or LightCycler® 480 Probes Master
A 85 bp long fragment from the blaIMP gene is amplified with specific primers and detected with a LC640 labeled hydrolysis probe. The current kit will detect most described IMP variants but will miss IMP-9,16,18,22, and 25. The positive control included in the kit is identical with the IMP-1 sequence. In a German study including more than 60,000 samples no IMP positive cases had been detected.
In a German study including 60,000 samples only two cases were detected. Since PCR eventually reports results which can be not verified in culture only negative results should be considered as true.
This assay detects 10 genome equivalent copies or less per reaction (plasmid DNA dilution).
Sample Material and Extraction
Typical samples are from feces, rectal swabs or bacterial culture. See ModularDx Extraction Protocols.